Department of Botany, Awadhesh Pratab Singh University, Rewa, Madya Pradesh, 486003, India.
Laboratory of Green Synthesis, Department of Chemistry, University of Allahabad, Allahabad, 211002, India.
Microb Pathog. 2018 May;118:211-213. doi: 10.1016/j.micpath.2018.03.040. Epub 2018 Mar 22.
In the present study petroleum ether, chloroform and methanolic extracts of Oscimum sanctum were prepared using soxhlet extractor. The extracts were evaluated for antibacterial activity against one Gram positive (Staphylococcus aureus) and one Gram negative (Escherichia coli) strain. The activity of the extracts was compared with the known antibacterial drugs, Oflaxacin and Penicillin G. Disc diffusion method revealed good antibacterial activity of the chloroform and methanol extracts compared to the petroleum ether extract. Methanolic extract was found to be most active against both Staphylococcus aureus and Escherichia coli bacterial strains. The zone of inhibition of methanolic extract against Staphylococcus aureus and Escherichia coli at 50 mg concentration was 16.0 and 18.0 mm, respectively. Chloroform extract also exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains. The zone of inhibition of chloroform extract against Staphylococcus aureus and Escherichia coli bacterial strains was 8.0 and 6.0 mm, respectively. The zone of inhibition of the drug Penicillin G against Escherichia coli and Staphylococcus aureus was18.0 and 17.0 mm, respectively. Oflaxacin showed zone of inhibition to be 19.0 and 20.0 against Escherichia coli and Staphylococcus aureus, respectively. The petroleum ether extract exhibited no activity against any of the two tested bacterial strains. These findings suggest that methanolic extract of Oscimum sanctum has anti-bacterial potential and therefore should be investigated for phytochemistry.
在本研究中，采用索氏提取器制备了罗勒（Oscimum sanctum）的石油醚、氯仿和甲醇提取物。评估了这些提取物对一株革兰氏阳性（金黄色葡萄球菌）和一株革兰氏阴性（大肠杆菌）菌株的抗菌活性。将提取物的活性与已知的抗菌药物奥沙西林和青霉素 G 进行了比较。圆盘扩散法显示，与石油醚提取物相比，氯仿和甲醇提取物具有良好的抗菌活性。甲醇提取物对金黄色葡萄球菌和大肠杆菌的活性最强。50mg 浓度的甲醇提取物对金黄色葡萄球菌和大肠杆菌的抑菌圈直径分别为 16.0 和 18.0mm。氯仿提取物对金黄色葡萄球菌和大肠杆菌也表现出良好的抗菌活性。氯仿提取物对金黄色葡萄球菌和大肠杆菌的抑菌圈直径分别为 8.0 和 6.0mm。青霉素 G 对大肠杆菌和金黄色葡萄球菌的抑菌圈直径分别为 18.0 和 17.0mm。奥沙西林对大肠杆菌和金黄色葡萄球菌的抑菌圈直径分别为 19.0 和 20.0mm。石油醚提取物对两种测试的细菌菌株均无活性。这些发现表明，罗勒的甲醇提取物具有抗菌潜力，因此应进行植物化学研究。
