Grönroos M, Mäenpää J, Kangas L, Erkkola R, Paul R, Grenman S
Department of Obstetrics and Gynecology, University Central Hospital, Turku, Finland.
Ann Chir Gynaecol Suppl. 1987;202:76-9.
The sensitivity to medroxyprogesterone acetate (MPA) and tamoxifen (T) and their combination was assayed in 13 patients with untreated endometrial cancer by an in vitro ATP-bioluminescence method. The method measures the levels of adenosinetriphosphate (ATP), the basic energy source of living cells. A tumor was considered to respond to the drug, if the proportion of living cells after manipulation was 50% or less from unmanipulated control culture. Estrogen (ER) and progesterone (PR) receptors were assayed by the DCC-method and the results calculated by Scatchard-analysis. ER (greater than or equal to 3 fmol/kg cytosol protein) was present in all tumors and PR (greater than or equal to 10 fmol/kg cytosol protein) in 85% of the tumors. The response rate in vitro to MPA was 67% (8 out of 12 tumors), to T 18% (2 out of 11) and to their combination 69% (9 out of 13). The G1 tumors responded statistically significantly better to MPA (p less than 0.01) and MPA + T (p less than 0.02) as compared to T. MPA produced higher cell kill of G1 than G2 tumors (p less than 0.05). The ER content correlated with the effect of MPA in vitro in 67%, with the effect of T in 18% and with that of their combination in 69% of the tumors. The PR content correlated with the effects of MPA in vitro in 83%, with the effect of T in 36% and with that of their combination in 54% of the tumors. It was concluded that the in vitro ATP-bioluminescence method provides valuable information besides steroid receptor determinations for sensitivity testing of endometrial cancer to hormones.
采用体外ATP生物发光法检测了13例未经治疗的子宫内膜癌患者对醋酸甲羟孕酮（MPA）、他莫昔芬（T）及其联合用药的敏感性。该方法可测量活细胞的基本能量来源三磷酸腺苷（ATP）的水平。如果处理后活细胞比例相对于未处理的对照培养物为50%或更低，则认为肿瘤对该药物有反应。采用葡聚糖包被活性炭法（DCC法）检测雌激素（ER）和孕激素（PR）受体，并通过Scatchard分析计算结果。所有肿瘤均存在ER（≥3 fmol/kg胞浆蛋白），85%的肿瘤存在PR（≥10 fmol/kg胞浆蛋白）。体外对MPA的反应率为67%（12例肿瘤中有8例），对T为18%（11例中有2例），对其联合用药为69%（13例中有9例）。与T相比，G1期肿瘤对MPA（p<0.01）和MPA+T（p<0.02）的反应在统计学上显著更好。MPA对G1期肿瘤的细胞杀伤作用高于G2期肿瘤（p<0.05）。67%的肿瘤中ER含量与MPA的体外作用相关，18%与T的作用相关，69%与它们联合用药的作用相关。83%的肿瘤中PR含量与MPA的体外作用相关，36%与T的作用相关，54%与它们联合用药的作用相关。得出结论，体外ATP生物发光法除了类固醇受体测定外，还为子宫内膜癌激素敏感性检测提供了有价值的信息。
