Yu Xiaofen, Luo Qingchen, Huang Kaixun, Yang Guangxiao, He Guangyuan
The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan, China.
School of Chemistry and Chemical Engineering, Huazhong University of Science & Technology, Wuhan, China.
Front Plant Sci. 2018 Mar 15;9:326. doi: 10.3389/fpls.2018.00326. eCollection 2018.
Microelement contents and metabolism are vitally important for cereal plant growth and development as well as end-use properties. While minerals phytotoxicity harms plants, microelement deficiency also affects human health. Genetic engineering provides a promising way to solve these problems. As plants vary in abilities to uptake, transport, and accumulate minerals, and the key enzymes acting on that process is primarily presented in this review. Subsequently, microelement function and biosafety assessment of transgenic cereal plants have become a key issue to be addressed. Progress in genetic engineering of cereal plants has been made with the introduction of quality, high-yield, and resistant genes since the first transgenic rice, corn, and wheat were born in 1988, 1990, and 1992, respectively. As the biosafety issue of transgenic cereal plants has now risen to be a top concern, many studies on transgenic biosafety have been carried out. Transgenic cereal biosafety issues mainly include two subjects, environmental friendliness and end-use safety. Different levels of gene confirmation, genomics, proteomics, metabolomics and nutritiomics, absorption, metabolism, and function have been investigated. Also, the different levels of microelement contents have been measured in transgenic plants. Based on the motivation of the requested biosafety, systematic designs, and analysis of transgenic cereal are also presented in this review paper.
微量元素含量及代谢对于谷类作物的生长发育以及最终用途特性至关重要。虽然矿物质的植物毒性会损害植物,但微量元素缺乏也会影响人类健康。基因工程为解决这些问题提供了一条有前景的途径。由于植物在吸收、运输和积累矿物质的能力方面存在差异,本文主要介绍了作用于该过程的关键酶。随后,转基因谷类作物的微量元素功能及生物安全性评估已成为亟待解决的关键问题。自1988年、1990年和1992年分别诞生第一株转基因水稻、玉米和小麦以来,谷类作物基因工程在引入品质、高产和抗性基因方面取得了进展。随着转基因谷类作物的生物安全性问题现已成为首要关注焦点,人们开展了许多关于转基因生物安全性的研究。转基因谷类生物安全性问题主要包括两个方面,即环境友好性和最终用途安全性。人们对基因鉴定、基因组学、蛋白质组学、代谢组学和营养组学、吸收、代谢及功能的不同层面进行了研究。此外,还测定了转基因植物中不同水平的微量元素含量。基于所需生物安全性的动机,本文还介绍了转基因谷类的系统设计与分析。