中华乳菇（Fisch.）胱氨酸丰富基因 llCDT1 增强酵母细胞和拟南芥对镉的耐受性。

Iris lactea var. chinensis (Fisch.) cysteine-rich gene llCDT1 enhances cadmium tolerance in yeast cells and Arabidopsis thaliana.

机构信息

Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China.

College of Horticulture, Nanjing Agricultural University, Nanjing 210014, China.

出版信息

Ecotoxicol Environ Saf. 2018 Aug 15;157:67-72. doi: 10.1016/j.ecoenv.2018.03.059. Epub 2018 Mar 30.

DOI:10.1016/j.ecoenv.2018.03.059

PMID:29605645

Abstract

UNLABELLED

IlCDT1, a cysteine-rich protein, was isolated from Iris lactea var. chinensis (Fisch.) (I. lactea var. chinensis). Its transcription was up-regulated by the exogenous application of Cd. The truncated IlCDT1 (25-54) containing 14 Cys residues confers Cd tolerance to yeast as the intact IlCDT1, indicating that Cys residues are required for Cd tolerance presumably by chelating Cd. When the gene was constitutively expressed in A. thaliana, root length of transgenic lines was longer than that of wild-type under 100 μM or 200 μM Cd stress. However, Cd absorption in wild-type was more than in two trangenic lines under 100 μM Cd exposure. IlCDT1 may directly bind Cd, through chelating Cd and avoiding the Cd uptake into the cells. Together, IlCDT1 may be a promising gene for the Cd tolerance improvement.

SUMMARY

Cysteine-rich gene llCDT1 enhances cadmium tolerance in yeast cells and Arabidopsis thaliana.

摘要

未标记

从鸢尾（中国）（鸢尾）中分离出富含半胱氨酸的蛋白质 IlCDT1。其转录受外源 Cd 的上调。含有 14 个半胱氨酸残基的截断 IlCDT1（25-54）赋予酵母对 Cd 的耐受性，就像完整的 IlCDT1 一样，表明半胱氨酸残基通过螯合 Cd 可能对 Cd 耐受性是必需的。当该基因在拟南芥中组成型表达时，与野生型相比，在 100µM 或 200µM Cd 胁迫下，转基因系的根长更长。然而，在 100µM Cd 暴露下，野生型的 Cd 吸收量超过了两个转基因系。IlCDT1 可能通过螯合 Cd 并避免 Cd 进入细胞来直接结合 Cd。总之，IlCDT1 可能是提高 Cd 耐受性的有前途的基因。