Negishi K, Teranishi T, Kuo C H, Miki N
Department of Neurophysiology, School of Medicine, University of Kanazawa, Japan.
Vision Res. 1987;27(8):1237-41. doi: 10.1016/0042-6989(87)90199-4.
The localization of structures immunoreactive to antisera against opsin (OPS; a specific marker for rods) and against visinin (VIS; a specific marker for cones in the vertebrate retina) was investigated in the retina of the river lamprey (Lampetra japonica) by means of fluorescence microscopy. Different immunoreactive structures were found with the two antisera. A vitreadly located structure in the outer nuclear layer displayed OPS-like immunoreactivity, while a scleradly located one exhibited VIS-like reactivity. Thus, the two antisera applied to the retina of the river lamprey permit the distinguishing of rod and cone immunoreactive photoreceptors such as occurs typically in all vertebrate classes. The former appears in the outer segments of a type of rod cells, and the latter in the cell bodies and axons of cones.
通过荧光显微镜研究了抗视蛋白抗血清（OPS；视杆细胞的特异性标记物）和抗视锥蛋白抗血清（VIS；脊椎动物视网膜中视锥细胞的特异性标记物）免疫反应性结构在日本七鳃鳗视网膜中的定位。用这两种抗血清发现了不同的免疫反应性结构。在外核层中位于玻璃体侧的结构显示出OPS样免疫反应性，而位于巩膜侧的结构则表现出VIS样反应性。因此，应用于日本七鳃鳗视网膜的这两种抗血清能够区分视杆和视锥免疫反应性光感受器，这在所有脊椎动物类群中通常都会出现。前者出现在一种视杆细胞的外段，而后者出现在视锥细胞的胞体和轴突中。
