微小 RNA-520a-3p 通过抑制高迁移率族蛋白 B1（HMGB1）抑制非小细胞肺癌。
MicroRNA-520a-3p suppresses non-small-cell lung carcinoma by inhibition of High Mobility Group Box 1 (HMGB1).
机构信息
Department of Respiratory, Linyi People's Hospital, Linyi, China.
出版信息
Eur Rev Med Pharmacol Sci. 2018 Mar;22(6):1700-1708. doi: 10.26355/eurrev_201803_14583.
OBJECTIVE
Currently, pathogenesis of non-small cell lung carcinoma (NSCLC) is still unknown and the treatment is far from ideal. Therefore, we investigated the effect of inhibiting microRNA-520a-3p in NSCLC cells.
MATERIALS AND METHODS
NCI-H157 cells were treated with microRNA-520a-3p analogs for 48 h, or microRNA-520a-3p analogs and its inhibitor, for a total of 48 h. Many tests were performed, including MTT, flow cytometry, wound healing assay and transwell assay. The tumor model was established, and HMGB1 mRNA was detected by RT-PCR. Protein levels of HMGB1, MMP-2, MMP-9, Bcl-2, Bax, and Caspase-3 were assessed by Western Blot.
RESULTS
microRNA-520a-3p could significantly inhibit the proliferation, migration and invasion of NCI-H157 cells, inducing their apoptosis. microRNA-520a-3p inhibited tumor growth and decreased the mRNA and protein levels of HMGB1. Additionally, it decreased the Bcl-2/Bax ratio, MMP-2 and MMP-9 expression, and increased caspase-3 expression.
CONCLUSIONS
microRNA-520a-3p exhibited an effective inhibition on NCI-H157 tumor growth likely by inhibiting HMGB1 expression.
目的
目前，非小细胞肺癌（NSCLC）的发病机制尚不清楚，治疗远非理想。因此，我们研究了抑制非小细胞肺癌细胞中 microRNA-520a-3p 的作用。
材料和方法
用 microRNA-520a-3p 类似物处理 NCI-H157 细胞 48 小时，或用 microRNA-520a-3p 类似物及其抑制剂共处理 48 小时。进行了许多测试，包括 MTT、流式细胞术、划痕愈合试验和 Transwell 试验。建立肿瘤模型，通过 RT-PCR 检测 HMGB1 mRNA。通过 Western Blot 评估 HMGB1、MMP-2、MMP-9、Bcl-2、Bax 和 Caspase-3 的蛋白水平。
结果
microRNA-520a-3p 可显著抑制 NCI-H157 细胞的增殖、迁移和侵袭，诱导其凋亡。microRNA-520a-3p 抑制肿瘤生长，降低 HMGB1 mRNA 和蛋白水平。此外，它降低了 Bcl-2/Bax 比值、MMP-2 和 MMP-9 表达，并增加了 caspase-3 表达。
结论
microRNA-520a-3p 对 NCI-H157 肿瘤生长具有有效抑制作用，可能通过抑制 HMGB1 表达。
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