Lima Jaqueline Ataíde Silva, Rezende Hanstter Hallison Alves, Rocha Tamires Mariana Dias Damas, Castro Ana Maria de
Laboratório de Estudos da Relação Parasito-Hospedeiro, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás - UFG, Goiânia, GO, Brasil.
Rev Bras Parasitol Vet. 2018 Jan-Mar;27(1):95-98. doi: 10.1590/S1984-29612018004. Epub 2018 Feb 19.
Cats are carriers of zoonotic agents to humans, including intestinal parasites. The purpose of this study was to analyze the accuracy of different laboratory methods for the diagnosis of intestinal parasites. Fecal samples were processed by the Willis, Sheather, Faust and Hoffman-Janer-Pons-Lutz (HJPL) methods. Accuracy analysis was performed determining the sensitivity, specificity, positive and negative predictive value and Kappa. A total of 149 fecal samples were collected, 65 from stray cats and 84 from domiciled cats. The prevalence of intestinal parasites in stray cats was 60% while in domiciled cats it was 17%. In the analysis of accuracy, the techniques that showed the greatest accuracy for Ancylostomids were Willis and Faust, for Cystoisospora spp. Sheather with Faust or HPJL, and Toxoplasma gondii/Hammondia hammondi the association between Willis and Faust. Therefore, for a reliable evaluation of the prevalence of intestinal parasites, at least two different techniques should be used in parasitological exams of feces.
猫是包括肠道寄生虫在内的人畜共患病原体的携带者。本研究的目的是分析诊断肠道寄生虫的不同实验室方法的准确性。粪便样本采用威利斯法、谢瑟法、福斯特法和霍夫曼 - 亚内尔 - 庞斯 - 卢茨(HJPL)法进行处理。通过确定敏感性、特异性、阳性和阴性预测值以及kappa值进行准确性分析。共收集了149份粪便样本,其中65份来自流浪猫,84份来自家养猫。流浪猫肠道寄生虫的患病率为60%,而家养猫为17%。在准确性分析中,对钩虫显示出最高准确性的技术是威利斯法和福斯特法,对等孢球虫属是谢瑟法与福斯特法或HPJL法,对刚地弓形虫/哈蒙德弓形虫是威利斯法和福斯特法的联合使用。因此,为了可靠评估肠道寄生虫的患病率,在粪便寄生虫学检查中应至少使用两种不同的技术。