IBMM, UMR 5247, Univ. Montpellier, CNRS, ENSCM, Montpellier, France.
Org Biomol Chem. 2018 May 2;16(17):3181-3188. doi: 10.1039/c8ob00328a.
The synthesis and the impact of a disulfide bridge between 2'-O-positions of two adjacent nucleotides in an RNA duplex and in the loop of RNA hairpins are reported. The incorporation of this 2',2'-disulfide (S-S) bridge enabled thermal and enzymatic stabilization of the hairpin depending on its position in the loop. The influence of the disulfide bridge on RNA folding was studied at the HIV Dimerization Initiation Site (DIS) as an RNA sequence model. We have shown that this S-S bridge locked the hairpin form, whereas the extended duplex form was generated after the reduction of the disulfide bond in the presence of tris(2-carboxyethyl)phosphine or glutathione. Thus, the S-S bridge can be useful for understanding RNA folding; an RNA molecular beacon locked by an S-S bridge was also investigated as a sensor for the detection of glutathione.
报道了在 RNA 双链体的 2'-O-位置和 RNA 发夹环中相邻两个核苷酸之间形成二硫键（S-S）的合成及其影响。该 2'，2'-二硫键（S-S）桥的引入能够根据其在环中的位置实现发夹结构的热稳定性和酶稳定性。该二硫键对 HIV 二聚化起始位点（DIS）中 RNA 折叠的影响作为 RNA 序列模型进行了研究。我们已经表明，该 S-S 桥锁定了发夹构象，而在三（2-羧乙基）膦或谷胱甘肽存在下还原二硫键后，会生成延伸的双链构象。因此，S-S 桥可用于理解 RNA 折叠；我们还研究了一种由 S-S 桥锁定的 RNA 分子信标作为谷胱甘肽检测的传感器。
