State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 73 Huanghe Road, Harbin, 150090, PR China; Advanced Water Management Centre, The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia.
Advanced Water Management Centre, The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia.
J Environ Manage. 2018 Jul 1;217:825-831. doi: 10.1016/j.jenvman.2018.04.029. Epub 2018 Apr 24.
This study investigated the role of COD/N ratio on the start-up and performance of an upflow microaerobic sludge reactor (UMSR) treating piggery wastewater at 0.5 mgO/L. At high COD/N ratio (6.24 and 4.52), results showed that the competition for oxygen between ammonia-oxidizing bacteria, nitrite-oxidizing bacteria and heterotrophic bacteria limited the removal of nitrogen. Nitrogen removal efficiency was below 40% in both scenarios. Decreasing the influent COD/N ratio to 0.88 allowed achieving high removal efficiencies for COD (∼75%) and nitrogen (∼85%) due to the lower oxygen consumption for COD mineralization. Molecular biology techniques showed that nitrogen conversion at a COD/N ratio 0.88 was dominated by the anammox pathway and that Candidatus Brocadia sp. was the most important anammox bacteria in the reactor with a relative abundance of 58.5% among the anammox bacteria. Molecular techniques also showed that Nitrosomonas spp. was the major ammonia-oxidiser bacteria (relative abundance of 86.3%) and that denitrification via NO and NO also contributed to remove nitrogen from the system.
本研究考察了 COD/N 比对处理猪场废水的上流式微需氧污泥反应器（UMSR）启动和性能的影响，O2 浓度为 0.5mg/L。在高 COD/N 比（6.24 和 4.52）下，结果表明氨氧化菌、亚硝酸盐氧化菌和异养菌之间对氧气的竞争限制了氮的去除。在这两种情况下，氮去除效率均低于 40%。将进水 COD/N 比降低至 0.88，由于 COD 矿化过程的耗氧量较低，可实现 COD（75%）和氮（85%）的高去除效率。分子生物学技术表明，在 COD/N 比为 0.88 时，氮的转化主要由厌氧氨氧化途径主导，而在反应器中，Candidatus Brocadia sp. 是最重要的厌氧氨氧化菌，相对丰度为 58.5%。分子技术还表明，Nitrosomonas spp. 是主要的氨氧化菌（相对丰度为 86.3%），通过 NO 和 NO 的反硝化也有助于从系统中去除氮。
