Maier A, Leberer E, Pette D
Department of Cell Biology and Anatomy, University of Alabama, Birmingham 35294.
Histochemistry. 1988;88(3-6):273-6. doi: 10.1007/BF00570284.
Sarcoplasmic reticulum (SR) Ca2+-pumping ATPase (Ca-ATPase) and calsequestrin (CaS) were visualized by indirect immunofluorescence at the polar regions of adult rat, rabbit and cat intrafusal fibers. The immunohistochemical reaction products were regarded as histochemical markers of the SR and as valid indicators of the distribution of the two Ca2+-sequestering proteins. Static nuclear bag2 fibers displayed lower levels of both Ca-ATPase and CaS than the other two intrafusal fiber types. Nuclear chain fibers presented the highest Ca-ATPase levels and, together with dynamic nuclear bag1 fibers, they also exhibited relatively high amounts of CaS. The level of Ca-ATPase was lower in bag 1 fibers than in nuclear chain fibers, but not as low as in bag2 fibers. The comparatively high levels of Ca-ATPase and CaS seen in nuclear chain fibers coincided with their reported faster contractile speeds compared to nuclear bag fibers.
通过间接免疫荧光法在成年大鼠、兔和猫梭内肌纤维的两极区域观察到肌浆网（SR）钙泵ATP酶（Ca-ATP酶）和肌集钙蛋白（CaS）。免疫组化反应产物被视为SR的组织化学标志物以及两种钙螯合蛋白分布的有效指标。静态核袋2纤维的Ca-ATP酶和CaS水平均低于其他两种梭内肌纤维类型。核链纤维的Ca-ATP酶水平最高，并且与动态核袋1纤维一起，它们也表现出相对较高量的CaS。袋1纤维中的Ca-ATP酶水平低于核链纤维，但不像袋2纤维中那么低。核链纤维中观察到的相对较高水平的Ca-ATP酶和CaS与报道的它们相比于核袋纤维更快的收缩速度相一致。
