Department of Chemistry, Faculty of Arts and Sciences, Bulent Ecevit University, 67100, Zonguldak, Turkey.
Appl Biochem Biotechnol. 2018 Nov;186(3):547-562. doi: 10.1007/s12010-018-2749-8. Epub 2018 Apr 19.
A new derivate of 4-bromo-1,8-naphthalic anhydride and its quaternized analogue have been prepared and characterized. The interactions of both derivatives with human telomere quadruplex-DNA and ds-DNA have been comparatively studied by UV-visible (UV-Vis), fluorescent intercalator displacement assays, competition dialysis, circular dichroism (CD), agarose gel electrophoresis, and polyacrylamide gel electrophoresis. The results show that both derivatives can stabilize G-quadruplexes DNA, and they show different binding affinities for G-quadruplexes-DNA and ds-DNA. All spectroscopic studies have shown that the derivatives have a modest selectivity for G-quadruplex versus ds-DNA.
一种新的 4-溴-1,8-萘二甲酸酐衍生物及其季铵化类似物已经被制备和表征。通过紫外可见（UV-Vis）光谱、荧光嵌入剂置换实验、竞争透析、圆二色性（CD）、琼脂糖凝胶电泳和聚丙烯酰胺凝胶电泳等方法比较研究了两种衍生物与人端粒四链体-DNA 和 ds-DNA 的相互作用。结果表明，两种衍生物都可以稳定 G-四链体 DNA，它们与 G-四链体-DNA 和 ds-DNA 的结合亲和力不同。所有光谱研究表明，该衍生物对 G-四链体与 ds-DNA 具有适度的选择性。
