Höper J, Kessler M
Institut für Physiologie und Kardiologie, Universität Erlangen-Nürnberg, FRG.
Int J Microcirc Clin Exp. 1988 Mar;7(2):155-68.
Isolated rat livers were perfused with a haemoglobin-free Krebs-Ringer solution containing 35 g albumin/1. During a 3 hour perfusion total flow, O2-uptake rate and local O2-supply remained constant. During the same period of time the ATP-content increased from a value of 0.59 +/- 0.17 mumol/g to 1.81 +/- 0.42 mumol/g. The low initial value was due to the ischaemic period during preparation of the organ. The glycogen content increased by 13.4 mg/g, indicating that the organ did not lose the ability for glyconeogenesis. In addition to an increase in glycogen, there was a continuous increase in the glucose concentration of the perfusate, while lactate was taken up by the organ. The results indicate that the isolated organ can be perfused for at least 3 hours without development of disturbances in local O2-supply.
用不含血红蛋白的Krebs-Ringer溶液（含35 g白蛋白/1）灌注离体大鼠肝脏。在3小时的灌注过程中，总流量、氧气摄取率和局部氧气供应保持恒定。在同一时间段内，ATP含量从0.59±0.17 μmol/g增加到1.81±0.42 μmol/g。初始值较低是由于器官制备过程中的缺血期。糖原含量增加了13.4 mg/g，表明该器官没有丧失糖异生能力。除了糖原增加外，灌注液中的葡萄糖浓度持续升高，而乳酸则被器官摄取。结果表明，离体器官可以灌注至少3小时而不会出现局部氧气供应紊乱。
