Water Environ Res. 2018 May 1;90(5):424-430. doi: 10.2175/106143017X15131012152979.
This study was carried out to analyze the heavy metals biosorption potential of bacteria isolated from soil contaminated with electroplating industrial effluents. Bacterial isolates were screened for their multi-metal biosorption potential against copper, nickel, lead, and chromium. Bacterial isolate CU4A showed the maximum uptake of copper, nickel, lead, and chromium in aqueous solution, with a biosorption efficiency of 87.16 %, 79.62%, 84.92%, and 68.12%, respectively. The bacterial strain CU4A was identified as Bacillus cereus, following 16S rRNA gene sequence analysis. The surface chemical functional groups of bacterial biomass were identified by Fourier transform infrared (FTIR) spectroscopy as hydroxyl, carboxyl, amine, and halide, which may be involved in the biosorption of heavy metals. Analysis with scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) confirmed the adsorption of metals on the bacterial cell mass. The results of this study are significant and could be further investigated for the removal of heavy metals from contaminated environments.
本研究旨在分析从电镀工业废水污染土壤中分离出的细菌对重金属的生物吸附潜力。对细菌分离物进行了筛选,以研究其对铜、镍、铅和铬的多金属生物吸附潜力。细菌分离物 CU4A 在水溶液中对铜、镍、铅和铬的最大吸收率分别为 87.16%、79.62%、84.92%和 68.12%。通过 16S rRNA 基因序列分析,将该细菌菌株 CU4A 鉴定为解淀粉芽孢杆菌。通过傅里叶变换红外(FTIR)光谱鉴定了细菌生物质的表面化学官能团,包括羟基、羧基、胺和卤化物,这些官能团可能参与了重金属的生物吸附。扫描电子显微镜(SEM)和能谱(EDS)分析证实了金属在细菌细胞质量上的吸附。本研究结果意义重大,可进一步研究用于从污染环境中去除重金属。