利用ISSR标记揭示伊朗L.的遗传多样性与结构。
Genetic diversity and structure of L. in Iran as revealed by ISSR markers.
作者信息
Ahmadi Maryam, Saeidi Hojjatollah
机构信息
Department of Biology, University of Isfahan, Isfahan, 81746-73441 Iran.
出版信息
Physiol Mol Biol Plants. 2018 May;24(3):483-491. doi: 10.1007/s12298-018-0518-3. Epub 2018 Apr 10.
L. (caper bush) is an economically and ecologically important perennial shrub that grows across different regions of Iran. In this study, the genetic diversity and population structure of Iranian genepool of is evaluated using Inter Simple Sequence Repeat (ISSR) markers. Using 10 ISSR primers, 387 DNA fragments (bands) were amplified from the genomic DNA of 92 individuals belonging to twenty-one populations of . , of which 378 (97.7%) were polymorphic. High level of genetic diversity (percentage of polymorphic loci = 98.2%, h = 0.1382, I = 0.243), high genetic differentiation (G = 0.5234) and low gene flow (Nm = 0.4553) among populations were observed. Caper bush populations were divided into 4 groups in the dendrogram, PCoA plot and Bayesian clustering results, mostly corresponded to their geographic regions. The results showed that there are value in sampling Iranian caper bush populations to look for valuable alleles for use in plant breeding programs.
刺山柑（刺山柑属）是一种在经济和生态方面都很重要的多年生灌木，生长在伊朗的不同地区。在本研究中，利用简单重复序列区间（ISSR）标记对伊朗刺山柑基因库的遗传多样性和种群结构进行了评估。使用10个ISSR引物，从属于刺山柑21个种群的92个个体的基因组DNA中扩增出387个DNA片段（条带）。其中，378个（97.7%）具有多态性。观察到种群间存在高水平的遗传多样性（多态位点百分比=98.2%，h=0.1382，I=0.243）、高遗传分化（G=0.5234）和低基因流（Nm=0.4553）。在聚类图、主坐标分析图和贝叶斯聚类结果中，刺山柑种群被分为4组，大多与其地理区域相对应。结果表明，对伊朗刺山柑种群进行采样以寻找用于植物育种计划的有价值等位基因具有重要意义。
Zotero 插件