电纺植物黏液纳米纤维作为细胞增殖的生物相容性支架。
Electrospun plant mucilage nanofibers as biocompatible scaffolds for cell proliferation.
机构信息
Department of Biological Systems Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA.
Department of Animal Science, University of Wisconsin-Madison, Madison, WI 53706, USA.
出版信息
Int J Biol Macromol. 2018 Aug;115:1218-1224. doi: 10.1016/j.ijbiomac.2018.04.129. Epub 2018 Apr 25.
Electrospun nanofibers (ESNFs) were prepared from mucilage isolated from chan and linaza beans and mozote stem commercially available in Costa Rica. Poly(vinyl alcohol) (PVA) was used as an aiding agent. Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) were prepared and adjusted to be similar in viscosity and electrical conductivity suitable for electrospinning. Morphology of the ESNFs was examined using scanning electron microscopy (SEM). Fourier transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies were used to characterize chemical composition and thermal characteristics of the nanofibers (NFs). The ability of the NFs to support fibroblast cell proliferation was investigated in vitro using the optimized mucilage/PVA solutions. Results show plant mucilage-based ESNFs are well-suited for fibroblast cell growth, significantly better than ESNFs of PVA; and the mucilage of chan beans is better than those of mozote and linaza for supporting cell proliferation.
静电纺丝纳米纤维（ESNFs）是由哥斯达黎加市售的 Chan 豆和 Linaza 豆的粘胶以及 Mozote 茎中分离出的粘胶与聚乙烯醇（PVA）混合制备而成。粘胶/PVA 混合溶液具有不同的体积比（100:0、80:20、60:40、40:60、20:80 和 0:100），并进行了调节，使其具有相似的粘度和电导率，适合静电纺丝。使用扫描电子显微镜（SEM）观察 ESNFs 的形态。傅里叶变换红外光谱仪（FTIR）和差示扫描量热法（DSC）研究用于表征纳米纤维（NFs）的化学组成和热特性。使用优化的粘胶/PVA 溶液体外研究 NF 对成纤维细胞增殖的支持能力。结果表明，基于植物粘胶的 ESNFs 非常适合成纤维细胞生长，明显优于 PVA 的 ESNFs；而 Chan 豆的粘胶比 Mozote 和 Linaza 的粘胶更有利于细胞增殖。
Zotero 插件