Discipline of Molecular Medicine and Pathology, College of Medicine and Public Health, Flinders University, Adelaide, South Australia, Australia.
Discipline of Molecular Medicine and Pathology, College of Medicine and Public Health, Flinders University, Adelaide, South Australia, Australia.
Exp Hematol. 2018 Jul;63:28-32.e1. doi: 10.1016/j.exphem.2018.04.003. Epub 2018 Apr 26.
The cryopreservation of peripheral blood mononuclear cells (PBMCs) is a routine research laboratory process, enabling long-term storage of primary patient blood samples. Retrospective analysis of these samples has the potential to identify markers that may be associated with prognosis and response to treatment. To draw valid biological conclusions from this type of analysis, it is essential to ensure that any observed changes are directly related to the pathology of the disease rather than the preservation process itself. Therefore, we have investigated 15 cell surface markers that are relevant to chronic lymphocytic leukemia (CLL) on matched fresh and thawed samples to determine the effect of cryopreservation on their detection. We found that the number of CLL cells positive for the markers CD22, CD40, CD49d, CD54, CD69, and CXCR3 was decreased significantly after cryopreservation. In addition, the mean fluorescence intensity of 10 of the 15 markers changed significantly after cryopreservation. These findings demonstrate that care must be taken when interpreting this type of analysis on thawed samples.
外周血单个核细胞 (PBMCs) 的冷冻保存是常规的研究实验室过程，能够长期储存原发性患者的血液样本。对这些样本的回顾性分析有潜力确定与预后和治疗反应相关的标志物。为了从这种类型的分析中得出有效的生物学结论，必须确保观察到的任何变化都直接与疾病的病理相关，而不是与保存过程本身相关。因此，我们研究了与慢性淋巴细胞白血病 (CLL) 相关的 15 个细胞表面标志物，以确定冷冻保存对它们检测的影响。我们发现，冷冻保存后，标记物 CD22、CD40、CD49d、CD54、CD69 和 CXCR3 阳性的 CLL 细胞数量显著减少。此外，冷冻保存后 15 个标记物中有 10 个的平均荧光强度发生了显著变化。这些发现表明，在解释解冻样本上的这种类型的分析时必须谨慎。
