Liang Chunsu, Sun Hui, Meng Xiangjun, Yin Lei, Fawcett J Paul, Yu Huaidong, Liu Ting, Gu Jingkai
Research Center for Drug Metabolism, School of Life Sciences, Jilin University, 2699 Qianjin Street, Changchun 130012, China.
School of Pharmacy, University of Otago, Dunedin, New Zealand.
Acta Pharm Sin B. 2018 Mar;8(2):228-234. doi: 10.1016/j.apsb.2018.01.011. Epub 2018 Mar 10.
Because many therapeutic agents are contaminated by epimeric impurities or form epimers as a result of metabolism, analytical tools capable of determining epimers are increasingly in demand. This article is a proof-of-principle report of a novel DMS-MS/MS method to separate and simultaneously quantify epimers, taking PGF2 and its 8-epimer, 8--PGF2, as an example. Good accuracy and precision were achieved in the range of 10-500 ng/mL with a run time of only 1.5 min. Isopropanol as organic modifier facilitated a good combination of sensitivity and separation. The method is the first example of the quantitation of epimers without chromatographic separation.
由于许多治疗剂被差向异构体杂质污染，或在代谢过程中形成差向异构体，因此对能够测定差向异构体的分析工具的需求日益增加。本文是以PGF2及其8-差向异构体8-epi-PGF2为例，介绍一种用于分离和同时定量差向异构体的新型DMS-MS/MS方法的原理验证报告。在10-500 ng/mL范围内实现了良好的准确度和精密度，运行时间仅为1.5分钟。异丙醇作为有机改性剂促进了灵敏度和分离效果的良好结合。该方法是无需色谱分离即可定量差向异构体的首个实例。
