Morin M D, Lang-Ouellette D, Lyons P J, Crapoulet N, Morin P
Department of Chemistry and Biochemistry, Universite de Moncton, New Brunswick, Canada.
Atlantic Cancer Research Institute, Pavillon Hotel-Dieu, New Brunswick, Canada.
Cryo Letters. 2017 Jul/Aug;38(4):269-277.
Mammalian hibernation is a fascinating phenomenon that involves multiple molecular and biochemical changes to proceed. While the molecular picture associated with torpor has become clearer in recent years, the function of non-coding RNAs, and especially of microRNAs, solicited during this process is not well understood.
To better characterize a signature of cold torpor-associated miRNAs in the hibernating thirteen-lined ground squirrel Ictidomys tridecemlineatus.
Next-generation sequencing and qRT-PCR approaches were conducted in euthermic and hibernating ground squirrel liver tissues.
This high-throughput approach notably revealed modulation during hibernation of various miRNAs previously associated with lipid metabolism, glucose metabolism and antioxidant responses such as miR-145a-3p, miR-22-3p and miR-25-3p, respectively.
Overall, these results present a group of miRNAs differentially expressed in hibernating ground squirrel liver and provide additional knowledge on the underlying functions of these small non-coding molecules during cold torpor.
哺乳动物的冬眠是一种引人入胜的现象，在此过程中会发生多种分子和生化变化。尽管近年来与蛰伏相关的分子情况已变得更加清晰，但在此过程中被激活的非编码RNA，尤其是微小RNA的功能仍未得到充分了解。
更好地描绘冬眠的十三条纹地松鼠（Ictidomys tridecemlineatus）中与冷蛰伏相关的微小RNA特征。
在正常体温和冬眠状态的地松鼠肝脏组织中采用新一代测序和定量逆转录-聚合酶链反应方法。
这种高通量方法显著揭示了冬眠期间各种先前分别与脂质代谢、葡萄糖代谢和抗氧化反应相关的微小RNA（如miR-145a-3p、miR-22-3p和miR-25-3p）的调节情况。
总体而言，这些结果呈现了一组在冬眠地松鼠肝脏中差异表达的微小RNA，并为这些小非编码分子在冷蛰伏期间的潜在功能提供了更多知识。
