Xu H, Yang J J, Wang C H, Guo E Y, Yang N H, Zhao Q
Clin Exp Obstet Gynecol. 2016;43(4):573-577.
To explore the function of Wnt/β-catenin signal pathway on promoting the adhesion, invasion, and metastasis of endometriosis tissues by analyzing its effects on the expressions of matrix metalloproteinase-7 (MMP-7) and vascular endothelial growth factor in en- dometriosis.
Endometriosis nude mice models were included. Small RNA interference technology was used to block Wnt/β-catenin signal pathway. HE staining technique was adopted to observe the difference of pathological morphology among groups. The immunohistochemistry and real-time quantitative PCR were perfonned to analyze the expressions of $-catenin, MMP-7 and VEGF from pro- tein and mRNA levels.
Whether the Wnt/β-catenin signal pathway was blocked or not had little effect on the pathological mor- phology of lesions. The expressions of P-catenin, MMP-7 and VEGF in siRNA group were much lower than those in negative control group and control group (p < 0.05), while there was no statistical significance in the difference of expressions between negative control group and control group (p > 0.05).
Blocking of Wnt/β-catenin signal pathway caused the decrease of MMP-7 and VEGF expressions, in- dicating that Wnt/β-catenin signal pathway plays an important role in the adhesion, invasion, and metastasis of endometriosis tissues.
通过分析Wnt/β-连环蛋白信号通路对子宫内膜异位症组织中基质金属蛋白酶-7（MMP-7）和血管内皮生长因子表达的影响，探讨其在促进子宫内膜异位症组织黏附、侵袭和转移中的作用。
纳入子宫内膜异位症裸鼠模型。采用小RNA干扰技术阻断Wnt/β-连环蛋白信号通路。采用苏木精-伊红（HE）染色技术观察各组病理形态学差异。采用免疫组织化学和实时定量PCR从蛋白质和mRNA水平分析β-连环蛋白、MMP-7和血管内皮生长因子（VEGF）的表达。
阻断或未阻断Wnt/β-连环蛋白信号通路对病变的病理形态学影响不大。小干扰RNA（siRNA）组中β-连环蛋白、MMP-7和VEGF的表达明显低于阴性对照组和对照组（p<0.05），而阴性对照组和对照组之间的表达差异无统计学意义（p>0.05）。
阻断Wnt/β-连环蛋白信号通路导致MMP-7和VEGF表达降低，表明Wnt/β-连环蛋白信号通路在子宫内膜异位症组织的黏附、侵袭和转移中起重要作用。
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