Jang Yura, Lee Jeongae, Chung Hesson
Center for Neuro-Medicine, Korea Institute of Science and Technology.
Molecular Recognition Research Center, Korea Institute of Science and Technology.
Anal Sci. 2018;34(5):623-626. doi: 10.2116/analsci.17N034.
We developed a colorimetric method for measuring the amount of oil in mouse stool after co-administering an oil-soluble dye. When the amount of oil in the feces calculated from the amounts of Sudan III and Oil Red O was plotted against the amount of oil detected by liquid chromatography-mass spectrometry, the graph was linear, showing a one-to-one correlation between two analyses. This method may be utilized to determine the efficacy of lipase inhibitors, or to assess fat malabsorption in vivo.
我们开发了一种比色法，用于在共同施用油溶性染料后测量小鼠粪便中的油量。当根据苏丹III和油红O的量计算出的粪便中油量与通过液相色谱 - 质谱法检测到的油量作图时，该图呈线性，表明两种分析之间存在一对一的相关性。该方法可用于确定脂肪酶抑制剂的功效，或评估体内脂肪吸收不良情况。
