Department of Anatomy and Cell Biology College of Veterinary Medicine and Research Institute for Veterinary Science Seoul National University Seoul Korea.
Department of Anatomy College of Veterinary Medicine Konkuk University Seoul Korea.
Brain Behav. 2018 Mar 22;8(5):e00961. doi: 10.1002/brb3.961. eCollection 2018 May.
Genetic background influences neurotransmitter expression and function of the hippocampus. Genetic background influences the phenotype of the hippocampus, but expression of neuroglia in hippocampus has not been well established dependent on various mouse strains.
In this study, we investigated the effects of genetic background on cell population of astrocytes and microglia in eight widely used inbred strains (C57BL/6J, A/J, BALB/c, C3H/HeJ, FVB, 129/SvJ, DBA/1, and DBA/2) and one outbred strain (ICR).
In all mouse strains, glial fibrillary acidic protein (GFAP)-immunoreactive astrocytes and ionized calcium-binding adaptor molecule 1 (Iba-1)-immunoreactive microglia were found in almost all layers of hippocampal CA1-4 regions and the dentate gyrus.
We observed significant differences in the number of astrocytes and microglia. In the CA1 and CA3 regions, the number of GFAP-immunoreactive astrocytes was highest in the C3H/HeJ strain, and lowest in the 129/SvJ and FVB strains. In the polymorphic layer of the dentate gyrus, the number was highest in the DBA/1 strain and lowest in the 129/SvJ strain. Among the nine mouse strains, the number of Iba-1-immunoreactive microglia was highest in the CA1 and CA3 regions in the ICR and in the dentate gyrus of the C57BL/6 strain. The CA1 region of the FVB strain and the CA3 region and dentate gyrus of DBA/2 had the lowest number of Iba-1-immunoreactive microglia.
These results suggest that the numbers of astrocytes and microglia differ depending on the mouse strain and these differences may be related to strain-dependent function of astrocytes.
遗传背景会影响海马体的神经递质表达和功能。遗传背景会影响海马体的表型，但各种小鼠品系中海马体神经胶质的表达尚未得到很好的证实。
在这项研究中，我们研究了遗传背景对 8 种常用近交系（C57BL/6J、A/J、BALB/c、C3H/HeJ、FVB、129/SvJ、DBA/1 和 DBA/2）和 1 种远交系（ICR）中海马体星形胶质细胞和小胶质细胞群体的影响。
在所有小鼠品系中，GFAP-免疫反应性星形胶质细胞和离子钙结合衔接分子 1（Iba-1）-免疫反应性小胶质细胞存在于海马体 CA1-4 区和齿状回的几乎所有层中。
我们观察到星形胶质细胞和小胶质细胞数量存在显著差异。在 CA1 和 CA3 区，C3H/HeJ 品系的 GFAP-免疫反应性星形胶质细胞数量最高，而 129/SvJ 和 FVB 品系的数量最低。在齿状回多形层，数量最高的是 DBA/1 品系，而 129/SvJ 品系的数量最低。在这 9 个小鼠品系中，ICR 中的 CA1 和 CA3 区以及 C57BL/6 品系的齿状回中 Iba-1-免疫反应性小胶质细胞数量最多。FVB 品系的 CA1 区、DBA/2 品系的 CA3 区和齿状回的 Iba-1-免疫反应性小胶质细胞数量最少。
这些结果表明，星形胶质细胞和小胶质细胞的数量因小鼠品系而异，这些差异可能与星形胶质细胞的品系依赖性功能有关。
