[Rat bone marrow mesenchymal stem cells promote apoptosis and inhibit proliferation and migration of RSC96 cells].

OBJECTIVE

To investigate the effect of rat bone marrow mesenchymal stem cells( BMSCs) on the proliferation and migration of rat RSC96 Schwann cells in vitro.

METHODS

BMSCs of Sprague Dawley( SD) rats were isolated by bone marrow cell adherent method. The morphology of the third generation of BMSCs was observed by inverted microscopy. The cell surface antigen makers CD19,CD34,CD73,CD105 were detected by flow cytometry. The third-passage BMSCs differentiated into osteoblasts and adipocytes after exposed to bone-inducing and lipid-inducing media for 3 and 2 weeks,respectively,and then were identified by oil red O and alkaline phosphatase staining. The third generation of BMSCs was co-cultured with RSC96 cells for 24 hours in 24 mm co-culture plate. The cell proliferation and colony formation of RSC96 cells were determined by MTT assay and plate cloning assay. The migration of RSC96 cells was detected by scratch and TranswellTMchamber assay. Bax and Bcl-2 expression levels of RSC96 cells were detect by Western blot analysis.

RESULTS

The third generation of BMSCs showed fusiform,spiral arrangement with similar cell size under an inverted microscope. The CD73 and CD105 expression levels of BMSCs were high,however,the CD34 and CD19 were low. BMSCs had large lipid droplets after adipogenic induction for 3 weeks; and the alkaline phosphatase staining of BMSCs was positive after osteogenic induction for 2 weeks. Compared to the control group,co-culture of RSC96 cells and BMSCs induced a significant decrease in the proliferation,colony formation and migration of RSC96 cells. Meanwhile,Bax protein expression increased,and Bcl-2protein expression decreases in RSC96 cells co-cultured with BMSCs.

CONCLUSION

BMSCs could promote the apoptosis and inhibit the proliferation and migration of RSC96 cells.