揭示 A-Raf 功能的互作组。
Revealing A-Raf functions through its interactome.
机构信息
Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, 727 Jingming South Road, Kunming 650500, China; Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 Jingming South Road, Kunming 650500, China.
Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 Jingming South Road, Kunming 650500, China; The First People's Hospital of Yunnan Province, 157 Jinbi Road, Kunming 650032, China.
出版信息
Biochim Biophys Acta Proteins Proteom. 2018 Aug;1866(8):849-856. doi: 10.1016/j.bbapap.2018.05.009. Epub 2018 May 16.
A-Raf is a member of the Raf kinase family. Unlike B-Raf and C-Raf, the functions of A-Raf remain obscure. To gain more insight into the biological functions of A-Raf, we investigated the A-Raf interactome using proteomics. We found 132 proteins that interact with A-Raf and confirmed the interaction of 12 of these proteins with A-Raf by western blotting. Our data suggested that A-Raf regulates apoptosis, RNA catabolism, GTPase activity, and cell adhesion by interacting with proteins located in different cellular compartments. We identified all ten hallmarks of cancer in these interacting proteins, suggesting that A-Raf is involved in carcinogenesis. Our results also indicated that A-Raf may play a role in different diseases and signaling pathways. These findings have identified potential regulators of A-Raf and provide a systemic insight into its biological functions.
A-Raf 是 Raf 激酶家族的一员。与 B-Raf 和 C-Raf 不同，A-Raf 的功能尚不清楚。为了更深入地了解 A-Raf 的生物学功能，我们使用蛋白质组学研究了 A-Raf 的相互作用组。我们发现了 132 种与 A-Raf 相互作用的蛋白质，并通过 Western blot 验证了其中 12 种蛋白质与 A-Raf 的相互作用。我们的数据表明，A-Raf 通过与位于不同细胞区室的蛋白质相互作用，调节细胞凋亡、RNA 分解代谢、GTPase 活性和细胞黏附。我们在这些相互作用的蛋白质中鉴定出了所有十个癌症的标志性特征，表明 A-Raf 参与了致癌作用。我们的结果还表明，A-Raf 可能在不同的疾病和信号通路中发挥作用。这些发现确定了 A-Raf 的潜在调节剂，并为其生物学功能提供了系统的见解。
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