CFR C07在深层发酵中生产γ-亚麻酸的工艺参数优化
Optimization of Process Parameters for the Production of γ-Linolenic Acid by CFR C07 in Submerged Fermentation.
作者信息
Varma Parvathy Sree, Chandrasekharan Salini, Venkateswaran Govindarajulu, Rajendran Santhosh, Mallapureddy Kiran Kumar, Pandey Ashok, Parameswaran Binod
机构信息
Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., 695019 Thiruvananthapuram, Kerala, India.
CSIR-Central Food Technological Research Institute, Kajjihundi, 570020 Mysore, Karnataka, India.
出版信息
Food Technol Biotechnol. 2018 Mar;56(1):96-100. doi: 10.17113/ftb.56.01.18.5464.
The production of γ-linolenic acid (GLA) by the fungus CFR C07 in submerged fermentation was studied. Culture parameters such as carbon source and incubation time were optimized. Four different extraction methods using solvents with acid washed sand, glass beads, lyophilization and Soxhlet extraction were evaluated for improved extraction of lipids from the fungal biomass after fermentation. The GLA production was initially optimized in 250-mL flask and the process was demonstrated in a 3-litre fermentor. The maximum GLA production was 882 mg/L in shake flask culture and 733 mg/L in the fermentor. The study shows that CFR C07 is a potent organism for the production of GLA under submerged conditions.
研究了真菌CFR C07在深层发酵中γ-亚麻酸（GLA）的生产情况。对碳源和培养时间等培养参数进行了优化。评估了四种不同的提取方法，分别是使用酸洗砂、玻璃珠、冻干和索氏提取的溶剂，以改进发酵后从真菌生物质中提取脂质的效果。GLA的生产最初在250毫升烧瓶中进行优化，并在3升发酵罐中进行了验证。摇瓶培养中GLA的最大产量为882毫克/升，发酵罐中为733毫克/升。该研究表明，CFR C07是深层条件下生产GLA的有效菌株。
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