一种对芝麻素三葡萄糖苷的β-1,2-糖苷键具有高度特异性的新型细菌β-葡萄糖苷酶的鉴定与表征。

Identification and characterization of a novel bacterial β-glucosidase that is highly specific for the β-1,2-glucosidic linkage of sesaminol triglucoside.

作者信息

Sakurai Akinori, Hongo Shuntaro, Nair Arun, Waki Toshiyuki, Oikawa Daiki, Nishio Takuma, Shimoyama Takefumi, Takahashi Seiji, Yamashita Satoshi, Nakayama Toru

机构信息

a Graduate School of Engineering , Tohoku University , Sendai , Japan.

b Kiyomoto Co. Ltd , Nobeoka , Japan.

出版信息

Biosci Biotechnol Biochem. 2018 Sep;82(9):1518-1521. doi: 10.1080/09168451.2018.1476123. Epub 2018 May 26.

DOI:10.1080/09168451.2018.1476123

PMID:29804519

Abstract

A gene (PSTG2) coding for a novel β-glucosidase belonging to glycoside hydrolase family 3 was identified in the vicinity of the previously identified β-glucosidase gene [sesaminol triglucoside (STG)-hydrolyzing β-glucosidase, PSTG1] in the genome of Paenibacillus sp. strain KB0549. Compared with PSTG1, recombinant PSTG2 more specifically acted on the β-1,2-glucosidic linkage of the STG molecule to transiently accumulate a larger amount of 6-O-(β-D-glucopyranosyl)-β-D-glucopyranosylsesaminol.

摘要

在芽孢杆菌属菌株KB0549的基因组中，在先前鉴定的β-葡萄糖苷酶基因[芝麻素三葡萄糖苷（STG）水解β-葡萄糖苷酶，PSTG1]附近鉴定出一个编码属于糖苷水解酶家族3的新型β-葡萄糖苷酶的基因（PSTG2）。与PSTG1相比，重组PSTG2对STG分子的β-1,2-糖苷键具有更特异性的作用，可瞬时积累大量的6-O-（β-D-吡喃葡萄糖基）-β-D-吡喃葡萄糖基芝麻素醇。