van Middlesworth L
Endocrinology. 1985 Feb;116(2):665-70. doi: 10.1210/endo-116-2-665.
Effects of KSCN on thyroidal iodine metabolism were studied in weanling mice fed a low iodide diet (LID) labeled continuously with 125I as iodide. The addition of KSCN (0.3 and 0.6 mg/g diet) resulted in the accumulation of an unusual iodinated protein within the follicles of the mouse thyroids. After 60 days, total thyroidal iodine was 4 times greater than in controls without thiocyanate. The iodinated protein was essentially insoluble at pH 8.0 and was very slowly released from the thyroids; it contained more MIT than DIT and little thyroid hormone. By use of three isotopes (125I, 127I, and 131I) and auto-radiographs, there were shown different iodinated proteins synthesized during high and low iodine intakes and coexistent but segregated in the colloid. Low doses of perchlorate or iodide inhibited or prevented accumulation of the essentially insoluble iodinated protein. It is suggested that when mouse thyroids are iodine depleted, thiocyanate increases the formation of an essentially insoluble iodinated thyroglobulin within the thyroid. Only a small fraction of the iodination may have occurred by this route, but the rate of formation exceeded the rate of release, so the product continuously accumulated.
在以碘化物形式持续用¹²⁵I标记的低碘饮食（LID）喂养的断乳小鼠中，研究了硫氰酸钾（KSCN）对甲状腺碘代谢的影响。添加KSCN（0.3和0.6毫克/克饮食）导致小鼠甲状腺滤泡内出现一种异常的碘化蛋白积累。60天后，甲状腺总碘含量比未添加硫氰酸盐的对照组高4倍。这种碘化蛋白在pH 8.0时基本不溶，从甲状腺中释放非常缓慢；它含有的一碘甲腺原氨酸（MIT）比二碘甲腺原氨酸（DIT）多，甲状腺激素含量很少。通过使用三种同位素（¹²⁵I、¹²⁷I和¹³¹I）以及放射自显影片显示，在高碘和低碘摄入期间合成了不同的碘化蛋白，它们共存于胶体中但相互分离。低剂量的高氯酸盐或碘化物可抑制或阻止这种基本不溶的碘化蛋白的积累。研究表明，当小鼠甲状腺碘缺乏时，硫氰酸盐会增加甲状腺内一种基本不溶的碘化甲状腺球蛋白的形成。可能只有一小部分碘化是通过这条途径发生的，但形成速率超过了释放速率，所以产物不断积累。
