钙调蛋白与培养的促性腺激素细胞中含有促性腺激素释放激素-受体复合物的膜片之间存在关联的证据。

Evidence for an association between calmodulin and membrane patches containing gonadotropin-releasing hormone--receptor complexes in cultured gonadotropes.

作者信息

Jennes L, Bronson D, Stumpf W E, Conn P M

出版信息

Cell Tissue Res. 1985;239(2):311-5. doi: 10.1007/BF00218009.

Abstract

participation of calmodulin, clathrin, and actin in receptor mediated endocytosis of gonadotropin-releasing hormone (GnRH) was studied in an in vitro system of dispersed pituitary cells with a triple staining procedure. Cells were incubated in D-Lys6-Pro9-Des10-GnRH-biotin and stained with avidin-peroxidase-diaminobenzidine. Calmodulin, clathrin, and actin as well as luteinizing hormone were identified by indirect immunofluorescence with FITC- and rhodamine-labeled second antibody. The results indicate a close spatial association of calmodulin, but not of clathrin and actin, with GnRH-containing plasma membrane patches.

摘要

采用三重染色法，在体外分散垂体细胞系统中研究了钙调蛋白、网格蛋白和肌动蛋白在促性腺激素释放激素（GnRH）受体介导的内吞作用中的参与情况。细胞用D-Lys6-Pro9-Des10-GnRH-生物素孵育，并用抗生物素蛋白-过氧化物酶-二氨基联苯胺染色。通过用异硫氰酸荧光素（FITC）和罗丹明标记的二抗进行间接免疫荧光鉴定钙调蛋白、网格蛋白、肌动蛋白以及促黄体生成素。结果表明，钙调蛋白与含GnRH的质膜斑块存在紧密的空间关联，而网格蛋白和肌动蛋白则不然。