基于木瓜蛋白酶@AuNCs-Cu 复合物的血清 D-青霉胺荧光开启检测法。
Fluorescence turn-on assay for detection of serum D-penicillamine based on papain@AuNCs-Cu complex.
机构信息
Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun Beiyijie, Beijing, 100190, PR China; Zhuhai College of Jilin University, Zhuhai, 519041, PR China.
Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun Beiyijie, Beijing, 100190, PR China; University of Chinese Academy of Sciences, 19A Yuquanlu, Beijing, 100049, PR China.
出版信息
Anal Chim Acta. 2018 Oct 5;1026:133-139. doi: 10.1016/j.aca.2018.04.014. Epub 2018 Apr 18.
Papain-stabilized gold nanoclusters (papain@AuNCs) with a red fluorescence emission at 639 nm have been generated successfully in aqueous solution. The fluorescence of papain@AuNCs could be quenched in the addition of Cu. Subsequently, a unique fluorescent probe based on papain@AuNCs-Cu complex has been constructed for sensitive, selective and "turn-on" detection of D-penicillamine (D-pen). The sensing probe has exhibited a remarkable fluorescence enhancement in the presence of D-pen, which can be measured ranging from 30.0 μM to 2.0 mM (r = 0.991) with the detection limit of 5.0 μM. Furthermore, the developed assay has been utilized to explore the metabolic process of D-pen in rats after intraperitoneal injection. As far as we concerned, the present study has reported the first analytical method for sensing serum D-pen in real time by using the fluorescent papain@AuNCs-Cu complex. Our strategy paves a way for digging up the biological and clinical application of the fluorescent AuNCs.
成功地在水溶液中生成了具有 639nm 红色荧光发射的木瓜蛋白酶稳定的金纳米簇（木瓜蛋白酶@AuNCs）。加入 Cu 后，木瓜蛋白酶@AuNCs 的荧光可以被猝灭。随后，基于木瓜蛋白酶@AuNCs-Cu 配合物构建了一种独特的荧光探针，用于灵敏、选择性和“开启”检测 D-青霉胺（D-pen）。在 D-pen 的存在下，传感探针表现出显著的荧光增强，可以在 30.0 μM 至 2.0 mM 的范围内进行测量（r=0.991），检测限为 5.0 μM。此外，该方法已用于探索 D-pen 在腹腔注射后的大鼠代谢过程。据我们所知，本研究通过使用荧光木瓜蛋白酶@AuNCs-Cu 配合物，首次报道了实时感应血清中 D-pen 的分析方法。我们的策略为挖掘荧光 AuNCs 的生物和临床应用开辟了道路。
Zotero 插件