Arnheiter H, Davis N L, Wertz G, Schubert M, Lazzarini R A
Cell. 1985 May;41(1):259-67. doi: 10.1016/0092-8674(85)90079-0.
We describe experiments with two monoclonal antibodies to the vesicular stomatitis virus (VSV) nucleocapsid protein N with strikingly different characteristics. Antibody 1 binds to nucleocapsids and probably the pool of free (unbound) N protein; it inhibits transcription in vitro, and when microinjected into cells, protects the cells against VSV. Antibody 2 binds poorly to nucleocapsids, does not inhibit transcription, but when microinjected into cells, binds selectively to the free N and delays the appearance of progeny virus. We have confirmed these results by analyzing the effect of these antibodies on in vitro genomic RNA synthesis. The results of both the in vivo and in vitro experiments show that the replication of the VSV genome is controlled by the availability of the nucleocapsid protein, even when the polymerase has access to the host factors and multiple phosphorylated forms of the NS protein thought to be involved in genomic RNA synthesis.
我们描述了针对水疱性口炎病毒（VSV）核衣壳蛋白N的两种单克隆抗体的实验，这两种抗体具有显著不同的特性。抗体1与核衣壳结合，可能还与游离（未结合）的N蛋白池结合；它在体外抑制转录，当显微注射到细胞中时，可保护细胞免受VSV感染。抗体2与核衣壳的结合较差，不抑制转录，但当显微注射到细胞中时，它选择性地与游离的N蛋白结合，并延迟子代病毒的出现。我们通过分析这些抗体对体外基因组RNA合成的影响证实了这些结果。体内和体外实验的结果均表明，即使聚合酶能够接触到宿主因子以及被认为参与基因组RNA合成的NS蛋白的多种磷酸化形式，VSV基因组的复制仍受核衣壳蛋白可用性的控制。
