Sun Bo, Zheng Ai-Hong, Zhang Fen, Wei Ke-Su, Chen Qing, Luo Ya, Zhang Yong, Wang Xiao-Rong, Lin Fu-Cheng, Yang Jun, Tang Hao-Ru
College of Horticulture, Sichuan Agricultural University, Chengdu 611130, People's Republic of China.
Zhengzhou Tobacco Research Institute, Zhengzhou 450001, People's Republic of China.
R Soc Open Sci. 2018 May 30;5(5):180261. doi: 10.1098/rsos.180261. eCollection 2018 May.
The metabolic profiles of tobacco leaves of two differential Chinese cultivars from different growing regions were analysed using gas chromatography-mass spectrometry (GC-MS). The results of principal component analysis, partial least-squares discriminant analysis and hierarchical cluster analysis showed significant differences in metabolome among three groups, identified 24 differential metabolites, and analysed the metabolic pathway in which the metabolites were involved. Among them, 13 metabolites were associated with geographical regions, including seven organic and fatty acids, four carbohydrates and two secondary metabolites. Four amino acids and two monosaccharides were associated with cultivars and the remaining five metabolites were associated with both. The relationships among the differential metabolites and the distinct characteristics of environment and cultivar were further discussed. In addition, correlation analysis indicated that most of the differential carbohydrates were negatively correlated with the differential amino acids and organic acids. Taken together, this study demonstrates the metabolite differences between two cultivars in different regions, and highlights the effect of environment and cultivar on tobacco leaf metabolism.
利用气相色谱-质谱联用仪（GC-MS）分析了来自不同种植区域的两个中国差异品种烟草叶片的代谢谱。主成分分析、偏最小二乘判别分析和层次聚类分析结果表明，三组之间的代谢组存在显著差异，鉴定出24种差异代谢物，并分析了这些代谢物所涉及的代谢途径。其中，13种代谢物与地理区域相关，包括7种有机和脂肪酸、4种碳水化合物和2种次生代谢物。4种氨基酸和2种单糖与品种相关，其余5种代谢物与两者都相关。进一步讨论了差异代谢物之间的关系以及环境和品种的独特特征。此外，相关性分析表明，大多数差异碳水化合物与差异氨基酸和有机酸呈负相关。综上所述，本研究证明了不同区域两个品种之间的代谢物差异，并突出了环境和品种对烟草叶片代谢的影响。
