Department of Biotechnology, College of Life Sciences and Biotechnology , Korea University , 145 Anam-ro , Seongbuk-gu, Seoul 02841 , Korea.
J Agric Food Chem. 2018 Jul 5;66(26):6814-6821. doi: 10.1021/acs.jafc.8b01792. Epub 2018 Jun 26.
3,6-Anhydro-l-galactose (l-AHG) is a bioactive constituent of agar polysaccharides. To be used as a cosmetic or pharmaceutical ingredient, l-AHG is more favorably prepared by enzymatic saccharification of agar using a combination of agarolytic enzymes. Determining the optimum enzyme combination from the natural repertoire is a bottleneck for designing an efficient enzymatic-hydrolysis process. We consider all theoretical enzymatic-saccharification routes in the natural agarolytic pathway of a marine bacterium, Saccharophagus degradans 2-40. Among these routes, three representative routes were determined by removing redundant enzymatic reactions. We simulated each l-AHG production route with simple kinetic models and validated the reaction feasibility with an experimental procedure. The optimal enzyme mixture (with 67.3% maximum saccharification yield) was composed of endotype β-agarase, exotype β-agarase, agarooligosaccharolytic β-galactosidase, and α-neoagarobiose hydrolase. This approach will reduce the time and effort needed for developing a coherent enzymatic process to produce l-AHG on a mass scale.
3,6-脱水-l-半乳糖（l-AHG）是琼脂多糖的一种生物活性成分。为了将其用作化妆品或药物成分，更倾向于使用琼脂裂解酶的组合通过酶法糖化琼脂来制备 l-AHG。从天然库中确定最佳的酶组合是设计高效酶解过程的瓶颈。我们考虑了海洋细菌 Saccharophagus degradans 2-40 天然琼脂裂解途径中的所有理论酶解途径。在这些途径中，通过去除冗余的酶反应确定了三个代表性途径。我们使用简单的动力学模型模拟了每个 l-AHG 生产途径，并通过实验程序验证了反应的可行性。最佳的酶混合物（最大糖化产率为 67.3%）由内切型β-琼脂酶、外切型β-琼脂酶、琼脂寡糖裂解β-半乳糖苷酶和α-新琼二糖水解酶组成。这种方法将减少开发大规模生产 l-AHG 的连贯酶解过程所需的时间和精力。
