Anderson J
Prog Clin Biol Res. 1985;178:497-504.
A blocking enzyme linked immunosorbent assay (ELISA) is described for the detection of group specific antibodies to bluetongue virus (BTV). The test is based upon the interruption of the reaction between BTV antigen and a group specific monoclonal antibody raised against BTV by the addition of serial dilutions of either bovine or ovine test sera. The presence of group specific antibodies to BTV in the test serum, results in inhibition of the monoclonal antibody which is detected as a reduction in the expected optical density (OD) reading. The test is capable of the detection of antibodies to all 22 types of BTV but unlike the agar gel precipitin (AGP) test does not show cross-reactions with antibodies to epizootic haemorragic disease (EHD) viruses. Antibodies to cellular proteins which give rise to complications in the AGP test and indirect ELISA are not detected by this assay. Preliminary studies indicate that due to its high specificity and sensitivity, the blocking ELISA is an ideal alternative to the AGP test. The use of such a monoclonal antibody would also facilitate the standardization of diagnostic testing between laboratories.
本文描述了一种用于检测蓝舌病病毒（BTV）群特异性抗体的阻断酶联免疫吸附测定（ELISA）。该检测基于通过添加牛或羊测试血清的系列稀释液来中断BTV抗原与针对BTV产生的群特异性单克隆抗体之间的反应。测试血清中存在BTV群特异性抗体，会导致单克隆抗体受到抑制，这表现为预期光密度（OD）读数降低。该检测能够检测所有22种BTV类型的抗体，但与琼脂凝胶沉淀（AGP）试验不同，它不会与流行性出血病（EHD）病毒的抗体发生交叉反应。该检测不会检测到在AGP试验和间接ELISA中引起并发症的细胞蛋白抗体。初步研究表明，由于其高特异性和敏感性，阻断ELISA是AGP试验的理想替代方法。使用这种单克隆抗体也将有助于实验室之间诊断检测的标准化。
