利用分散液相微萃取研究海藻补充剂对牛乳中生育酚浓度的影响。
Effect of seaweed supplementation on tocopherol concentrations in bovine milk using dispersive liquid-liquid microextraction.
机构信息
Pharmaceutical and Molecular Biotechnology Research Centre (PMBRC), Department of Science, Waterford Institute of Technology, Waterford, Ireland.
Eco-Innovation Research Centre, Department of Science, Waterford Institute of Technology, Waterford, Ireland.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Aug 15;1092:152-157. doi: 10.1016/j.jchromb.2018.06.013. Epub 2018 Jun 7.
A dispersive liquid-liquid microextraction (DLLME) method, combined with HPLC-UV detection, was developed for the extraction and preconcentration of δ-tocopherol from bovine milk. This method was used to study the effect of supplementing cow feed with the seaweed Ascophyllum nodosum on vitamin content in milk. The optimal experimental conditions were determined: 200 μL of chloroform (extraction solvent), 1.0 mL of ethanol (dispersive solvent), 5 mL of water (aqueous phase). Under these optimal conditions the DLLME method provided linearity in the range 0.01 μg/mL to 8 μg/mL with R values of 0.998. Limit of detection (LOD) was 0.01 μg/mL, while the enrichment factor was 89. Cow feed that was supplemented with Ascophyllum nodosum was shown to increase δ-tocopherol levels from 3.82 μg/mL to 5.96 μg/mL.
建立了一种分散液液微萃取（DLLME）方法，结合高效液相色谱-紫外检测，用于从牛奶中提取和浓缩 δ-生育酚。该方法用于研究在牛饲料中添加海藻裙带菜对牛奶中维生素含量的影响。确定了最佳实验条件：200 μL 氯仿（萃取溶剂）、1.0 mL 乙醇（分散溶剂）、5 mL 水（水相）。在这些最佳条件下，DLLME 方法在 0.01μg/mL 至 8μg/mL 的范围内呈现出线性关系，R 值为 0.998。检测限（LOD）为 0.01μg/mL，而富集因子为 89。添加裙带菜的牛饲料使 δ-生育酚水平从 3.82μg/mL 增加到 5.96μg/mL。
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