[Role of nicotinamide vadenine dinucleotide phosphate oxidase 4 in transforming growth factor-β-induced A549 cell migration].

OBJECTIVE

To explore the role of NADPH oxidase 4( NOX4) in transforming growth factor-β( TGF-β)-induced A549 cells migration.

METHODS

The A549 cells were allocated into five groups: TGF-β( stimulation) group, Normal control group, DPI( NOX4 inhibitor) group, TGF-β + DPI group, and DMSO( solvent control)group. The level of ROS were detected by flow cytometry instrument. The level of NOX4, snail and E-cadherin protein were detected by western blot. Use scratches experiment to express the change of A549 cells migration.

RESULTS

After the quantification by Quantity One software, the expression of NOX4 in TGF-β group is( 1. 80 ± 0. 07), the TGF-β +DPI group is( 0. 49 ± 0. 03)( F = 327. 071, P < 0. 001). The change of EMT related protein: the expression of snail protein in TGF-β group is( 9. 0 ± 0. 6), the TGF-β + DPI group is( 1. 8 ± 0. 3)( F = 119. 097, P < 0. 001), the expression of E-cadherin protein in TGF-β group is( 0. 5 ± 0. 1), the TGF-β + DPI group is( 3. 3 ± 0. 3)( F = 71. 063, P <0. 001). These aboveresult indicate that DPI can inhibit the expression of NOX4 and EMTprogress in A549 cells. Then TGF-β + DPI group compared with the TGF-β group, the scratch healing rate is decreased( F = 33. 899, P < 0. 001). It illustrates that DPI can inhibit migration ability of A549 cells.

CONCLUSION

After the NOX4 was inhibited by DPI, TGF-β-induced migration of A549 cells was inhibited. And this phenomenon is associated with the progress of TGF-β-induced EMT.