Okada H, Nawa M, Kimura J
J Clin Endocrinol Metab. 1985 Sep;61(3):404-7. doi: 10.1210/jcem-61-3-404.
The effect of estrogen on the induction of human endometrial peroxidase was studied in vitro. When 17 beta-estradiol was added to the incubation medium, endometrial peroxidase activity was markedly increased, and the highest enzymatic activities were reached at about 12 h of incubation. The ability of estrogen and related compounds to increase peroxidase activity at a concentration of 10(-7) M was 17 beta-estradiol greater than estrone greater than 17 alpha-ethynylestradiol greater than estriol = diethylstilbestrol. Mestranol, progesterone, and testosterone had almost no activity. The induction of peroxidase by 17 beta-estradiol was inhibited by actinomycin D or cycloheximide. This method may be useful for determining the direct estrogenic effect of compounds in human endometrium.
在体外研究了雌激素对人子宫内膜过氧化物酶诱导作用的影响。当将17β-雌二醇添加到孵育培养基中时，子宫内膜过氧化物酶活性显著增加，且在孵育约12小时时达到最高酶活性。雌激素及相关化合物在10(-7)M浓度下增加过氧化物酶活性的能力为17β-雌二醇＞雌酮＞17α-乙炔雌二醇＞雌三醇 = 己烯雌酚。炔雌醇、孕酮和睾酮几乎没有活性。放线菌素D或环己酰亚胺可抑制17β-雌二醇对过氧化物酶的诱导作用。该方法可能有助于确定化合物在人子宫内膜中的直接雌激素效应。
