使用布雷菲德菌素A和渥曼青霉素剖析拟南芥中与液泡运输相关的高尔基体后细胞器。

Use of Brefeldin A and Wortmannin to Dissect Post-Golgi Organelles Related to Vacuolar Transport in Arabidopsis thaliana.

作者信息

Takagi Junpei, Uemura Tomohiro

机构信息

Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.

出版信息

Methods Mol Biol. 2018;1789:155-165. doi: 10.1007/978-1-4939-7856-4_12.

DOI:10.1007/978-1-4939-7856-4_12

PMID:29916078

Abstract

Eukaryotic cells comprise various organelles surrounded by the membrane. Each organelle is characterized by unique proteins and lipids and has its own specific functions. Single membrane-bounded organelles, including the Golgi apparatus, endosomes, and vacuoles are connected by membrane trafficking. Identifying the organelle localization of a protein of interest is essential for determining the proteins physiological functions. Here, we describe methods for determining protein subcellular localization using the inhibitors brefeldin A and wortmannin in Arabidopsis thaliana.

摘要

真核细胞由被膜包围的各种细胞器组成。每个细胞器都以独特的蛋白质和脂质为特征，并具有其自身特定的功能。包括高尔基体、内体和液泡在内的单层膜细胞器通过膜运输相互连接。确定感兴趣蛋白质的细胞器定位对于确定该蛋白质的生理功能至关重要。在这里，我们描述了在拟南芥中使用布雷菲德菌素A和渥曼青霉素抑制剂来确定蛋白质亚细胞定位的方法。