ADP-核糖基转移酶抑制剂可增强用SV40 DNA转染后NIH3T3细胞的转化。
Inhibitors of ADP-ribosyl transferase enhance the transformation of NIH3T3 cells following transfection with SV40 DNA.
作者信息
Strain A J
出版信息
Exp Cell Res. 1985 Aug;159(2):531-5. doi: 10.1016/s0014-4827(85)80026-4.
The influence of inhibitors of the enzyme ADP-Ribosyl Transferase (ADPRT) upon the morphological transformation of NIH3T3 cells by calcium phosphate-SV40 DNA co-precipitates was examined. Marked enhancement in the frequency of foci formation was noted when 3-methoxybenzamide was added to cells either during or after exposure to SV40 DNA, but there was no effect when cells were only pretreated with the inhibitor. The greatest enhancement was observed when cells were exposed to inhibitor both during and after transfection with DNA. The involvement of ADPRT activity in the events during transfection of DNA into cells is not understood. These results, however, highlight the importance of the key regulatory molecule poly (ADP-ribose) in the events which occur within the nucleus and which lead to transformation with SV40 DNA.
研究了ADP - 核糖基转移酶（ADPRT）抑制剂对磷酸钙 - SV40 DNA共沉淀物诱导NIH3T3细胞形态转化的影响。当在暴露于SV40 DNA期间或之后向细胞中添加3 - 甲氧基苯甲酰胺时，观察到集落形成频率显著提高，但仅用抑制剂预处理细胞时则无效果。当细胞在DNA转染期间和之后都暴露于抑制剂时，观察到最大程度的增强。尚不清楚ADPRT活性在DNA转染入细胞过程中的作用。然而，这些结果突出了关键调节分子聚（ADP - 核糖）在细胞核内发生的、导致SV40 DNA转化的事件中的重要性。
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