Berman M I, Anand-Srivastava M B, Sairam M R
Mol Cell Endocrinol. 1985 Aug;42(1):49-57. doi: 10.1016/0303-7207(85)90006-1.
Basal and gonadotropin stimulated adenylate cyclase activity was assessed in testicular tissues obtained from men (20-80 years). A disparity was observed in the gonadotropin responsiveness of the human testicular adenylate cyclase system to hFSH and hCG stimulation. Of the tissues analyzed, 61% were FSH responsive and 22% showed low response to hCG. Forskolin, a diterpene which activates adenylate cyclase by a receptor independent mechanism, stimulated adenylate cyclase activity in the gonadotropin unresponsive tissues. This suggests that the tissue unresponsiveness is due to an uncoupling of the catalytic subunit of the adenylate cyclase. Several functional properties of the FSH responsive human testicular adenylate cyclase were investigated. hFSH and oFSH stimulated the enzyme activity in a concentration dependent manner. However, the hormone (DG-oFSH) in which 80% of the carbohydrate residues had been removed was inactive, despite its good binding ability to the FSH receptor. hFSH stimulated adenylate cyclase activity was inhibited by DG-oFSH but not by DG-hCG (deglycosylated hCG). The data demonstrates the existence of specific FSH and LH(hCG) receptors in human testicular membranes. The FSH receptors in some tissues are coupled to adenylate cyclase. The link between the FSH receptor and adenylate cyclase may be uncoupled in the presence of the deglycosylated form of oFSH resulting in a loss of hormone response.
对20至80岁男性的睾丸组织进行了基础和促性腺激素刺激的腺苷酸环化酶活性评估。观察到人类睾丸腺苷酸环化酶系统对hFSH和hCG刺激的促性腺激素反应存在差异。在分析的组织中，61%对FSH有反应，22%对hCG反应较低。福司可林是一种通过受体非依赖性机制激活腺苷酸环化酶的二萜类化合物，它刺激了促性腺激素无反应组织中的腺苷酸环化酶活性。这表明组织无反应性是由于腺苷酸环化酶催化亚基的解偶联。研究了FSH反应性人类睾丸腺苷酸环化酶的几种功能特性。hFSH和oFSH以浓度依赖性方式刺激酶活性。然而，去除了80%碳水化合物残基的激素（DG-oFSH）尽管与FSH受体具有良好的结合能力，但却无活性。hFSH刺激的腺苷酸环化酶活性受到DG-oFSH的抑制，但不受DG-hCG（去糖基化hCG）的抑制。数据表明人类睾丸膜中存在特异性FSH和LH（hCG）受体。某些组织中的FSH受体与腺苷酸环化酶偶联。在oFSH的去糖基化形式存在时，FSH受体与腺苷酸环化酶之间的联系可能会解偶联，导致激素反应丧失。
