菜豆根瘤菌共生质粒在三叶草根瘤菌和根癌农杆菌中的表达：与三叶草根瘤菌共生质粒的不兼容性

Expression of a Rhizobium phaseoli Sym plasmid in R. trifolii and Agrobacterium tumefaciens: incompatibility with a R. trifolii Sym plasmid.

作者信息

Hooykaas P J, den Dulk-Ras H, Regensburg-Tuïnk A J, van Brussel A A, Schilperoort R A

出版信息

Plasmid. 1985 Jul;14(1):47-52. doi: 10.1016/0147-619x(85)90031-9.

Abstract

Identification of the Sym plasmid in Rhizobium phaseoli strain RCC3622 is described. Introduction of this plasmid into R. trifolii or Agrobacterium tumefaciens strains resulted in bacteria capable of forming characteristic spherical root nodules on beans. This Sym plasmid, designated pSym9, was characterized as 275 MDa and nonconjugative. pSym9 was incompatible with the R. trifolii Sym plasmid pSym5, and carries genes determining a melanin-like black pigment. A second plasmid of 135 MDa, pRph3622a, was also transferred from R. phaseoli to R. trifolii and A. tumefaciens. Transconjugants carrying this plasmid did not form root nodules on beans. In contrast to other Rhizobium plasmids, pRph3622a was unstable in A. tumefaciens.

摘要

本文描述了菜豆根瘤菌RCC3622中Sym质粒的鉴定。将该质粒导入三叶草根瘤菌或根癌土壤杆菌菌株后，这些细菌能够在菜豆上形成特征性的球形根瘤。这种Sym质粒命名为pSym9，大小为275 MDa，是非接合型的。pSym9与三叶草根瘤菌的Sym质粒pSym5不亲和，并携带决定一种类黑色素黑色色素的基因。另一个135 MDa的质粒pRph3622a也从菜豆根瘤菌转移到了三叶草根瘤菌和根癌土壤杆菌中。携带该质粒的接合子在菜豆上不形成根瘤。与其他根瘤菌质粒不同，pRph3622a在根癌土壤杆菌中不稳定。