Department of Immunology and Cell Interaction, D.O. Ott Research Institute for Obstetrics, Gynecology and Reproduction, St. Petersburg, Russia.
Department of Histology, Cytology and Embryology, Pavlov First Saint Petersburg State Medical University, St. Petersburg, Russia.
Am J Reprod Immunol. 2018 Oct;80(4):e13016. doi: 10.1111/aji.13016. Epub 2018 Jun 29.
To determine if placental macrophages (Hofbauer cells) can synthesize and secrete cytokines of the IL-17 family throughout pregnancy and to reveal the patterns of cytokine expression in early and late gestation.
Macrophages were isolated from the first-trimester and term placental villous tissues from normal pregnancies. Basal and stimulated intracellular production of IL-17A, IL-17E, IL-17F as well as IL-17A secretion was quantified by flow cytometry and cytometric bead array, respectively. The expression of IL-17 and IL-23 receptors was determined on the surface of the placental macrophages by flow cytometry after antibody staining.
In early and late gestation, a substantial proportion of the placental macrophages synthesized IL-17A and IL-17F, but not IL-17E, as determined by intracellular staining of the cytokines. Neither the intracellular production nor the secretion of IL-17 was significantly affected by LPS stimulation and spontaneous labour. The level of secretion decreased slightly but significantly at term. The IL-23 receptor was absent on the surface of cells, whereas variable expression of the IL-17 receptor was observed.
Placental macrophages constitutively produced IL-17 at different gestational ages and represent thus a source of this cytokine in the human placenta. Patterns of the cytokine and receptor expression suggest that this cell population may participate in non-immune processes and contribute to the regulation of placental development and function.
确定胎盘巨噬细胞（Hofbauer 细胞）是否可以在整个怀孕期间合成和分泌白细胞介素-17 家族的细胞因子，并揭示早期和晚期妊娠中细胞因子表达的模式。
从正常妊娠的早期和足月胎盘绒毛组织中分离出巨噬细胞。通过流式细胞术和细胞计数珠阵列分别定量测定 IL-17A、IL-17E、IL-17F 的基础和刺激细胞内产生以及 IL-17A 的分泌。通过抗体染色后流式细胞术确定 IL-17 和 IL-23 受体在胎盘巨噬细胞表面的表达。
在早期和晚期妊娠中，通过细胞因子的细胞内染色确定，相当一部分胎盘巨噬细胞合成了 IL-17A 和 IL-17F，但不合成 IL-17E。LPS 刺激和自发性分娩对细胞内 IL-17 的产生和分泌均无显著影响。在足月时，分泌水平略有但显著下降。IL-23 受体不存在于细胞表面，而 IL-17 受体的表达则不同。
胎盘巨噬细胞在不同的妊娠年龄持续产生 IL-17，因此是人类胎盘内这种细胞因子的来源。细胞因子和受体表达模式表明，该细胞群可能参与非免疫过程，并有助于调节胎盘的发育和功能。
