正畸托槽粘接后基因编码雌激素受体（ESR1 和 ESR2）多态性与双酚 A 排泄水平的关系：初步研究。
Association between polymorphisms in genes encoding estrogen receptors (ESR1 and ESR2) and excreted bisphenol A levels after orthodontic bracket bonding: a preliminary study.
机构信息
Department of Pediatric Dentistry, Orthodontic Area, Ribeirão Preto Dental School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Department of Pediatric Dentistry, Ribeirão Preto Dental School, University of São Paulo, Av. Do Café s/n, Monte Alegre, Ribeirão Preto, São Paulo, 14040-904, Brazil.
出版信息
Prog Orthod. 2018 Jul 2;19(1):19. doi: 10.1186/s40510-018-0219-z.
BACKGROUND
Bisphenol A (BPA) is released from orthodontic composites used for bracket bonding. Genetic variations could modify the metabolism of this chemical within the organism. Considering that free BPA binds to estrogen receptors causing harmful effects to health, the present in vivo study aimed to evaluate the association between genetic polymorphisms in genes encoding estrogen receptors (ESR1 and ESR2) and excreted BPA levels in orthodontic patients.
METHODS
Quantification of BPA levels in the urine of 16 patients was performed in a gas chromatograph mass spectrometer before (T0), at 24 h (T1), and 1 week (T2) after bracket bonding. DNA was extracted from saliva, and one genetic polymorphism in ESR1 (rs2234693) and two in ESR2 (rs4986938 and rs1256049) were analyzed by real-time PCR. Increases in BPA levels in the urine at T1 and T2 were grouped according to the genotype, and mean differences were compared by unpaired T test or Mann-Whitney test according to the normality of the data. The established alpha was 5%.
RESULTS
BPA levels increased significantly at T1 and T2. There were no statistically significant differences in the increases in BPA levels according to the genotype for any genetic polymorphism (P > 0.05), at neither 24 h nor 1 week after bracket bonding.
CONCLUSIONS
The results suggested that there are no association between excreted BPA levels after bracket bonding and the evaluated genetic polymorphisms in ESR1 and ESR2. Further research should be performed in order to confirm these results.
背景
双酚 A（BPA）从用于托槽粘接的正畸复合材料中释放出来。遗传变异可能会改变该化学物质在体内的代谢。由于游离的 BPA 与雌激素受体结合，对健康造成有害影响，因此本体内研究旨在评估编码雌激素受体（ESR1 和 ESR2）的基因中的遗传多态性与正畸患者排泄的 BPA 水平之间的关系。
方法
在托槽粘接前（T0）、24 小时（T1）和 1 周（T2）时，使用气相色谱质谱仪对 16 名患者尿液中的 BPA 水平进行定量。从唾液中提取 DNA，并通过实时 PCR 分析 ESR1 中的一个遗传多态性（rs2234693）和 ESR2 中的两个遗传多态性（rs4986938 和 rs1256049）。根据基因型将 T1 和 T2 时尿液中 BPA 水平的增加分组，并根据数据的正态性，通过未配对 T 检验或曼-惠特尼检验比较平均值差异。建立的 alpha 值为 5%。
结果
T1 和 T2 时 BPA 水平显著增加。任何遗传多态性下，根据基因型，BPA 水平增加在 24 小时和 1 周后均无统计学差异（P>0.05）。
结论
结果表明，托槽粘接后 BPA 水平的排泄与 ESR1 和 ESR2 中评估的遗传多态性之间没有关联。为了确认这些结果，应该进行进一步的研究。
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