This study aims to investigate the etiologic spectrum features of hand, foot and mouth disease(HFMD)and the VP1 region of coxsackievirus A6(CV-A6)in the Anyang area of China during 2013.A total of 479 HFMD fecal specimens were identified using real-time RT-PCR. The positive specimens of the different serotypes and the proportion of each serotype were calculated. A total of 10 positive of CV-A6 specimens were amplified and sequenced to obtain the profile of the VP1 region. Based on the data of the VP1 sequences from the Anyang strains and other referenced strains deposited in the NCBI database, the similarities of the nucleotide and amino-acid sequences for the VP1 region, as well as phylogenetic analyses were performed by the BIOEIDT 7.2and MEGA 5.1software packages. The results revealed that 429 positive specimens of enterovirus, including 90 positive specimens of CV-A6 were identified. The CV-A6 specimens accounted for 20.98%of the total positive specimens.CV-A6 had become the second preponderant pathogen of the HFMD in the Anyang area during 2013.It was the first record of an enterovirus serotype of which was neither CV-A16 nor EV-A71,had become one of the primary pathogens associated with HFMD in the region. A great diversity of the nucleotide and amino-acid sequences between 10 Anyang strains and a prototype strain (Gdula) was found. Compared with the Henan strain(HN421),four Anyang sequences with nucleotide and amino-acid sequences exhibited greater diversity than the other six Anyang sequences. The phylogenetic analysis showed that all of 49CV-A6 strains could be clustered into four branches. Branch D could be further clustered into two sub-branches;6 Anyang sequences belonged to sub-branch D1,and the other four belonged to sub-branch D2.