Kang Peng-Liang, Zhang Hai-Han, Huang Ting-Lin, Chen Sheng-Nan, Shang Pan-Lu, Feng Ji, Jia Jing-Yu
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Huan Jing Ke Xue. 2018 May 8;39(5):2431-2437. doi: 10.13227/j.hjkx.201710229.
The effect of aerobic denitrifying bacteria is a hot topic in the field of water environment bioremediation. Aerobic denitrifier communities, H-30, X-10, and C-30, enriched by intermittent aeration, screened with screening culture media, and treated by ultrasonic waves, could perform high denitrification performance at the higher dissolved oxygen concentration of (7.2±0.6) mg ·L. The total nitrogen (TN) removal rate of aerobic denitrifier communities, H-30, X-10, and C-30, reached 83.04%, 83.40%, and 82.68%, respectively. There is lower nitrite accumulation during the process of denitrification. Illumina high-throughput DNA sequencing revealed that aerobic denitrifier compositions were significantly different among the three communities. The predominant strains of aerobic denitrifier communities, H-30, X-10, and C-30, were , , and , respectively. The proportion of in aerobic denitrifier communities H-30 and X-10 was almost the same, while was only detected in H-30. These three efficient aerobic denitrifier communities provide a bacterium source guarantee for polluted water bioremediation of lakes and reservoirs in cities.
好氧反硝化细菌的作用是水环境生物修复领域的一个热门话题。通过间歇曝气富集、用筛选培养基筛选并经超声波处理得到的好氧反硝化细菌群落H-30、X-10和C-30，在溶解氧浓度较高为(7.2±0.6)mg·L时可表现出较高的反硝化性能。好氧反硝化细菌群落H-30、X-10和C-30的总氮（TN）去除率分别达到83.04%、83.40%和82.68%。反硝化过程中亚硝酸盐积累较低。Illumina高通量DNA测序表明，这三个群落中的好氧反硝化细菌组成存在显著差异。好氧反硝化细菌群落H-30、X-10和C-30的优势菌株分别为、和。好氧反硝化细菌群落H-30和X-10中的比例几乎相同，而仅在H-30中检测到。这三个高效的好氧反硝化细菌群落为城市湖泊和水库污染水生物修复提供了菌种来源保障。
