Zhou Shuang Nan, Lyu Teng Teng, Chen Qi Cheng, Peng Rui Bing, Han Qing Xi, Jiang Xia Min
School of Marine Sciences, Ningbo University, Ningbo 315211, ZheJiang, China.
Ying Yong Sheng Tai Xue Bao. 2018 Jun;29(6):2059-2067. doi: 10.13287/j.1001-9332.201806.033.
We investigated the effects of lumination on hatching of fertilized eggs of Sepia pharaonis, to reveal the best light conditions for its embryonic development. A single-factor experiment was carried out to examine the effects of different light intensities (10, 30, 50, 70, 90 μmol·m·s) and different photoperiod L:D (24 h:0 h, 18 h:6 h, 12 h:12 h, 6 h:18 h, 0 h:24 h) on the embryonic development. The results showed that the effects of light intensity on the hatching rate, fractured yolk sac rate, incubation period, mass of newly hatched larvae and mantle length was significant. There was no significant effect on hatching period and survival rate after hatching 7 days. With the increases of light intensity, the hatching rate, incubation period, mass of newly hatched larvae and mantle length first increased and then decreased, while the fractured yolk sac rate gradually increased. The optimum light intensity was 30 μmol·m·s. Exposed to this light intensity, the hatching rate, fractured yolk sac rate, incubation period, hatching period, mass of newly hatched larvae, mantle length and survival rate after hatching 7 days were (90.0±4.1)%, (7.3±1.5)%, (25.50±0.35) d, (8.10±0.89) d, (0.213±0.011) g, (1.013±0.022) cm, (97.1±4.0)%, respectively. The effects of photoperiod on the hatching rate, incubation period, hatching period were significant, but there was no significant effect on fractured yolk sac rate, mass of newly hatched larvae, mantle length and survival rate after hatching 7 days. With the increases of illumination time, the hatching rate and hatching period first increased and then decreased. The optimum photoperiod was L:D (12 h:12 h). When exposed to this photoperiod environment, the hatching rate, fractured yolk sac rate, incubation period, hatching period, mass of newly hatched larvae, mantle length and survival rate after hatching 7 days were (88.7±1.8)%, (8.7±1.8)%, (25.00±0.50) d, (7.00±3.20) d, (0.209±0.005) g, (0.998±0.026) cm, (96.8±7.1)%, respectively. In conclusion, embryo hatchability of S. pharaonis preferred to low light intensity (30 μmol·m·s) and normal photoperiod L:D (12 h:12 h). In production practice, sunproof measures should be taken to keep the eggs in weak light condition.
我们研究了光照对法老乌贼受精卵孵化的影响，以揭示其胚胎发育的最佳光照条件。进行了单因素实验，以检验不同光照强度（10、30、50、70、90 μmol·m·s）和不同光周期L:D（24 h:0 h、18 h:6 h、12 h:12 h、6 h:18 h、0 h:24 h）对胚胎发育的影响。结果表明，光照强度对孵化率、破裂卵黄囊率、孵化期、初孵幼体质量和胴长有显著影响。对孵化期和孵化7天后的存活率没有显著影响。随着光照强度的增加，孵化率、孵化期、初孵幼体质量和胴长先增加后降低，而破裂卵黄囊率逐渐增加。最佳光照强度为30 μmol·m·s。在此光照强度下，孵化率、破裂卵黄囊率、孵化期、出膜期、初孵幼体质量、胴长和孵化7天后的存活率分别为（90.0±4.1）%、（7.3±1.5）%、（25.50±0.35）天、（8.10±0.89）天、（0.213±0.011）克、（1.013±0.022）厘米、（97.1±4.0）%。光周期对孵化率、孵化期、出膜期有显著影响，但对破裂卵黄囊率、初孵幼体质量、胴长和孵化7天后的存活率没有显著影响。随着光照时间的增加，孵化率和出膜期先增加后降低。最佳光周期为L:D（12 h:12 h）。在此光周期环境下，孵化率、破裂卵黄囊率、孵化期、出膜期、初孵幼体质量、胴长和孵化7天后的存活率分别为（88.7±1.8）%、（8.7±1.8）%、（25.00±0.50）天、（7.00±3.20）天、（0.209±0.005）克、（0.998±0.026）厘米、（96.8±7.1）%。总之，法老乌贼的胚胎孵化率偏好低光照强度（30 μmol·m·s）和正常光周期L:D（12 h:12 h）。在生产实践中，应采取防晒措施，使卵处于弱光条件下。
