IITB-Monash Research Academy, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India; Bioprocessing Laboratory, Centre for Technology Alternatives for Rural Areas (CTARA), Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
School of Chemistry, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
Bioresour Technol. 2018 Oct;266:322-334. doi: 10.1016/j.biortech.2018.06.072. Epub 2018 Jun 23.
An integrated biorefinery, incorporating hydrothermal processing of waste pomegranate peels (WPP), was proposed for the acid and organic solvent-free simultaneous recovery of pectin and phenolics with bioethanol production. The hydrothermal treatment (HT) was optimized using Box-Behnken design and the maximum recovery of pectin (18.8-20.9%) and phenolics (10.6-11.8%) were obtained by hydrothermal treatment at 115 °C for 40 min with a liquid-solid ratio of 10. The WPP pectin was characterized by IR, H NMR, and TGA which showed close similarity to commercial pectin. Depending on WPP cultivar type the degree of esterification, galacturonic acid content and molecular weight of pectin were in the range of 68-74%, 71-72%, and 131,137-141,538 Da, respectively. The recovered phenolics contained 57-60% punicalagin. Enzyme digestibility of WPP improved using HT with 177 g glucose produced per kg dry mass which was fermented to obtain 80 g ethanol with 88% of theoretical yield.
提出了一种集成的生物炼制厂，将水热处理废物石榴皮（WPP）与无酸和有机溶剂的果胶和酚类同时回收与生物乙醇生产相结合。使用 Box-Behnken 设计优化水热处理，在 115°C 下处理 40 分钟，液固比为 10，可获得最高的果胶（18.8-20.9%）和酚类（10.6-11.8%）回收率。WPP 果胶的特性通过 IR、H NMR 和 TGA 进行了表征，与商业果胶非常相似。根据 WPP 品种类型，果胶的酯化度、半乳糖醛酸含量和分子量分别在 68-74%、71-72%和 131,137-141,538 Da 的范围内。回收的酚类含有 57-60%的安石榴苷。使用 HT 处理可提高 WPP 的酶消化率，每公斤干质量可产生 177g 葡萄糖，这些葡萄糖可发酵获得 80g 乙醇，理论产率为 88%。
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