Department of Animal Sciences, Ohio Agricultural Research and Development Center, Wooster, OH 44691.
BIOMIN Holding GmbH, 3130 Getzersdorf, Austria.
Poult Sci. 2018 Oct 1;97(10):3510-3518. doi: 10.3382/ps/pey234.
This experiment was conducted to study the effects of drinking water supplementation of synbiotic product PoultryStar®sol (containing Lactobacillus reuteri, Bifidobacterium animalis, Pediococcus acidilactici, Enterococcus faecium, and fructo-oligosaccharide) in laying hens with and without a Salmonella challenge. A total of 384 one-day-old layer chicks were randomly distributed to the drinking water synbiotic supplementation or control groups. At 14 wk of age, the birds were vaccinated with a Salmonella vaccine, resulting in a 2 (control and synbiotic) X 2 (non-vaccinated and vaccinated) factorial arrangement. At 24 wk of age, half of the birds in the vaccinated groups and all the birds that were not vaccinated were challenged with Salmonella Enterica serotype Enteritidis, resulting in a 3 (vaccinated, challenged, vaccinated+challenged) X 2 (control and synbiotic) factorial arrangment. At 8 d post-Salmonella challenge, synbiotic supplementation decreased (P = 0.04) cecal S. Enteritidis in the challenge group compared to the un-supplemented challenge group. Birds that were supplemented with synbiotic in the vaccine + challenge group had significantly greater cecal B. animalis and P. acidilactici percentage at 10 d post-Salmonella challenge than the birds in the vaccine + challenge group without synbiotic supplementation. At 3 d post-Salmonella challenge, birds that were supplemented with synbiotic in the challenge group had significantly greater cecal L. reuteri percentage than the birds in the challenge group without synbiotic supplementation. At 17 d post-Salmonella challenge, synbiotic supplementation increased bile anti-Salmonella IgA in the challenge group compared to the birds in the challenge group without synbiotic supplementation by 76.0%. At 10 d (P < 0.01) and 30 d (P = 0.05) post-Salmonella challenge, synbiotic supplementation decreased LITAF mRNA expression compared to the un-supplemented groups. At 3 d post-Salmonella challenge, synbiotic supplementation in the vaccine group had longer jejunal villi compared to the vaccine group without synbiotic supplementation. This experiment demonstrated that drinking water supplementation of the synbiotic product evaluated can significantly manipulate immune response and intestinal microbiota of laying hens post-Salmonella challenge to handle the challenge effectively.
本实验旨在研究在有和没有沙门氏菌攻毒的情况下，给产蛋鸡饮用水中添加合生元产品 PoultryStar®sol（含有鼠李糖乳杆菌、动物双歧杆菌、乳酸片球菌、屎肠球菌和低聚果糖）对其产生的影响。选用 384 只 1 日龄蛋鸡雏随机分为饮用水中添加合生元组和对照组。14 周龄时，鸡群接种沙门氏菌疫苗，形成 2（对照组和合生元组）×2（未接种和接种）的析因设计。24 周龄时，接种组的一半鸡和所有未接种的鸡均进行了肠炎沙门氏菌攻毒，形成 3（接种、攻毒、接种+攻毒）×2（对照组和合生元组）的析因设计。攻毒后 8d，与未添加合生元的攻毒组相比，添加合生元降低了攻毒组盲肠中肠炎沙门氏菌的含量（P=0.04）。在疫苗+攻毒组中添加合生元的鸡在攻毒后 10d 盲肠中动物双歧杆菌和乳酸片球菌的比例显著高于未添加合生元的鸡。在攻毒后 3d，攻毒组中添加合生元的鸡盲肠中鼠李糖乳杆菌的比例显著高于未添加合生元的鸡。攻毒后 17d，与未添加合生元的攻毒组相比，添加合生元增加了攻毒组胆汁中抗沙门氏菌 IgA 的含量，增加了 76.0%。攻毒后 10d（P<0.01）和 30d（P=0.05），与未添加组相比，添加合生元降低了 LITAF mRNA 的表达。攻毒后 3d，与未添加合生元的疫苗组相比，添加合生元的疫苗组空肠绒毛更长。本实验表明，评估的合生元产品可以通过饮水方式添加到产蛋鸡中，在沙门氏菌攻毒后能显著调节免疫反应和肠道微生物群，从而有效应对攻毒。
