Regulation of rat brain angiotensin II (AII) receptors by intravenous AII and low dietary Na+.

Previous studies have shown the presence of specific AII receptors at several areas of the brain. The purpose of this study was to examine by radioreceptor assay the effect of intravenous AII infusion (5 or 25 ng/kg/min) and low dietary Na+ (less than 8 mmol/100 g) on AII receptors in five brain regions: the olfactory lobes (OLF), hypothalamus/thalamus/septum (HTS), midbrain (MID), cerebellum (CER) and medulla (MED). Scatchard analysis of binding data from control rats showed significant (P less than 0.01 ANOVA) differences between brain areas in both Ka (1.54 OLF, 1.87 HTS, 1.25 MID, 1.33 MED, 0.77 CER x 10(9) M-1) and Ro (321 OLF, 224 HTS, 203 MID, 145 MED, 41 CER fmol/g tissue). Following the i.v. infusion of AII for 4-7 days, marked changes were observed in the areas with a porous BBB, the HTS and MED. Both the Ka [3.20 (HTS) and 0.67 (MED) x 10(9) M-1] and Ro [116 (HTS) and 249 (MED) fmol/g tissue] changed. In addition, decreases in Ro were also observed in the OLF (241 fmol/g tissue) and CER (21 fmol/g tissue), areas which have not been considered as being accessible to blood-borne AII. A low Na+ diet for 21-30 days changed the Ka and Ro in all five regions but not in similar directions. Furthermore, with the exception of the OLF the direction of change was not similar to that caused by i.v. infusion of AII. It was concluded that AII receptor sites in the rat brain differ from each other in both receptor properties in their response to such regulatory factors as AII Na+ depletion.(ABSTRACT TRUNCATED AT 250 WORDS)