CTEN 诱导非小细胞肺癌细胞发生上皮间质转化（EMT）和转移。
CTEN induces epithelial-mesenchymal transition (EMT) and metastasis in non small cell lung cancer cells.
机构信息
Department of Oncology, The Jiangyin Hospital Affiliated to Medical College of Southeast University, Jiangyin, P.R.China.
Department of Obstetrics and Gynecology, Jiangyin Hospital of Traditional Chinese Medicine, Jiangyin, P.R.China.
出版信息
PLoS One. 2018 Jul 9;13(7):e0198823. doi: 10.1371/journal.pone.0198823. eCollection 2018.
To explore the effects and mechanism of CTEN (COOH-terminus tensin-like molecule) on EMT, cell migration and invasion of Human lung adenocarcinoma cells. The pCMV-vector, pCMV-CTEN, Control-shRNA, and CTEN-shRNA were transfected into A549 and NCI-H1299 cells by Lipofectamine 2000. Transforming growth factor-β1(TGF-β1)and epithelial-mesenchymal transition (EMT) -related biomarkers were detected by eliseand western blot. The migration and invasion ability of A549 cells and NCI-H1299 were examined by scratch-wound assay and transwell assay respectively. We found compare with control group, the expression of TGF-β and mesenchymal markers in CTEN overexpression group were increased, and the epithelial marker was decreased, which induced the EMT process. Meanwhile, scratch-woundassay showed that the migration efficiency of A549 and NCI-H1299 cells in CTEN overexpression group were higher than that in control group.Transwell assay demonstrated that the number of cells that migrated and invaded through the membrane were obviously more than those in control group.Furthermore, Knockdown of CTEN partially reversed transforming growth factor-β1(TGF-β1)-induced changes in EMT markers. In conclusion, CTEN activated the expression of TGF-β1, thereby prompting EMT in lung adenocareinma cancer cells.
探讨 CTEN（COOH 末端张力蛋白样分子）对人肺腺癌细胞 EMT、细胞迁移和侵袭的影响及其机制。通过 Lipofectamine 2000 将 pCMV-载体、pCMV-CTEN、对照-shRNA 和 CTEN-shRNA 转染到 A549 和 NCI-H1299 细胞中。通过 elise 和 Western blot 检测转化生长因子-β1（TGF-β1）和上皮-间充质转化（EMT）相关生物标志物。通过划痕试验和 Transwell 试验分别检测 A549 细胞和 NCI-H1299 的迁移和侵袭能力。我们发现与对照组相比，CTEN 过表达组中 TGF-β 和间充质标志物的表达增加，上皮标志物减少，诱导 EMT 过程。同时，划痕试验显示 CTEN 过表达组 A549 和 NCI-H1299 细胞的迁移效率高于对照组。Transwell 试验表明，穿过膜迁移和侵袭的细胞数明显多于对照组。此外，敲低 CTEN 部分逆转了 TGF-β1（TGF-β1）诱导的 EMT 标志物变化。总之，CTEN 激活 TGF-β1 的表达，从而促使肺腺癌细胞发生 EMT。
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