利用 [I]碘-DPA-713 SPECT/CT 对小鼠肺部异物反应进行成像。
Imaging Pulmonary Foreign Body Reaction Using [I]iodo-DPA-713 SPECT/CT in Mice.
机构信息
Center for Infection and Inflammation Imaging Research, Johns Hopkins University, 1550 Orleans Street, CRB-II, Rm 1.09, Baltimore, MD, 21287, USA.
Center for Tuberculosis Research, Johns Hopkins University, Baltimore, MD, USA.
出版信息
Mol Imaging Biol. 2019 Apr;21(2):228-231. doi: 10.1007/s11307-018-1249-0.
PURPOSE
Foreign body reactions elicit granulomatous inflammation composed of reactive macrophages. We hypothesized that [I]iodo-DPA-713 single-photon emission computed tomography (SPECT), a low-molecular-weight pyrazolopyrimidine ligand selectively trapped by phagocytes, could be used to detect foreign body reactions in a murine model.
PROCEDURES
C57BL/6 mice intratracheally inoculated with dextran beads, which developed foreign body lesions, were imaged after injection of [I]iodo-DPA-713 or DPA-713-IRDye800CW using SPECT and optical imaging, respectively.
RESULTS
Foreign body lesions were clearly observed in the lungs of the dextran-treated mice on computer tomography imaging and demonstrated significantly higher [I]iodo-DPA-713 uptake compared with control animals (p < 0.01). Ex vivo studies demonstrated granulomatous reactions in the lungs of dextran-treated mice and localization of DPA-713-IRDye800CW at the diseased sites confirming the imaging findings.
CONCLUSION
Radioiodinated DPA-713 may be used as a noninvasive biomarker for the detection of pulmonary foreign body reactions.
目的
异物反应会引发由反应性巨噬细胞组成的肉芽肿性炎症。我们假设 [I]碘-DPA-713 单光子发射计算机断层扫描 (SPECT)，一种被吞噬细胞选择性捕获的低分子量吡唑并嘧啶配体，可用于检测小鼠模型中的异物反应。
过程
用葡聚糖珠经气管内接种的 C57BL/6 小鼠发生异物病变，在注射 [I]碘-DPA-713 或 DPA-713-IRDye800CW 后，分别使用 SPECT 和光学成像进行成像。
结果
在计算机断层扫描成像上，明显观察到葡聚糖处理小鼠的肺部异物病变，与对照动物相比，[I]碘-DPA-713 的摄取明显更高（p<0.01）。离体研究表明，葡聚糖处理小鼠的肺部存在肉芽肿反应，DPA-713-IRDye800CW 定位于病变部位，证实了影像学发现。
结论
放射性碘标记的 DPA-713 可用作检测肺异物反应的非侵入性生物标志物。
Zotero 插件