用于曼氏血吸虫病诊断的PCR-ELISA实验室平台的开发。
Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA.
作者信息
Senra Carolina, Gomes Luciana Inácia, Siqueira Liliane Maria Vidal, Coelho Paulo Marcos Zech, Rabello Ana, Oliveira Edward
机构信息
Clinical Research and Public Politics in Infectious and Parasitic Diseases, Instituto René Rachou, Oswaldo Cruz Foundation (IRR/FIOCRUZ), Av. Augusto de Lima, 1715, Belo Horizonte, MG, 30190-002, Brazil.
Biology of the Schistosoma mansoni and its Interaction with Host, Instituto René Rachou, Oswaldo Cruz Foundation (IRR/FIOCRUZ), Av. Augusto de Lima, 1715, Belo Horizonte, MG, 30190-002, Brazil.
出版信息
BMC Res Notes. 2018 Jul 11;11(1):455. doi: 10.1186/s13104-018-3571-7.
OBJECTIVE
We developed a laboratorial platform to release a commercial platform used in the PCR-ELISA for the molecular diagnosis of schistosomiasis mansoni. On following, PCR-ELISA platform laboratorial was evaluated in 206 feces samples collected of individual living in a Brazilian low endemicity area.
RESULTS
The PCR-ELISA laboratorial platform indicated a prevalence rate of 25.2%, which was higher than the Kato-Katz technique (18.4%) and lower than the commercial platform (30.1%). Considering Kato-Katz technique as the reference, there were 97.4% and 91.1% of relative sensitivity and specificity rates, respectively. The laboratorial platform presented good precision, performance diagnostic, and can be used in replacement to the commercial platform for diagnosis of schistosomiasis by PCR-ELISA.
目的
我们开发了一个实验室平台，以推出用于曼氏血吸虫病分子诊断的PCR-ELISA商业平台。接下来，在巴西低流行区采集的206份粪便样本中对该PCR-ELISA实验室平台进行了评估。
结果
PCR-ELISA实验室平台显示的流行率为25.2%，高于加藤厚涂片法（18.4%）且低于商业平台（30.1%）。以加藤厚涂片法作为参考，相对灵敏度和特异度分别为97.4%和91.1%。该实验室平台具有良好的精密度和诊断性能，可用于替代商业平台进行PCR-ELISA法诊断血吸虫病。
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